Construct: ORF ccsbBroad304_10853
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007527.1_s304c1, BRDN0000404800
- Derived from:
- ccsbBroadEn_10853
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GOLGA2 (2801)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10853
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2801 | GOLGA2 | golgin A2 | NM_004486.5 | 100% | 100% | |
| 2 | human | 2801 | GOLGA2 | golgin A2 | NM_001366244.1 | 97.3% | 97.3% | 172_252del |
| 3 | human | 2801 | GOLGA2 | golgin A2 | XM_005251932.1 | 96.1% | 96.1% | 1_36del;479_480ins81 |
| 4 | human | 2801 | GOLGA2 | golgin A2 | NM_001366246.1 | 94.6% | 94.6% | 172_252del;524_525ins81 |
| 5 | human | 102466227 | MIR7162 | microRNA 7162 | NR_106985.1 | 1.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3036
- ORF length:
- 2970
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggaagaaacc cgacagagca aattggccgc agcgaagaaa aagttgagag 121 aatatcagca gaggaatagc cctggtgttc ctacaggagc gaaaaagaag aagaaaataa 181 aaaatggcag taaccctgag acaaccactt ctggtggttg ccactcacct gaggatacac 241 ccaaggacaa tgctgctact ctacaaccat ctgatgacac cgtgttacct ggcggtgtcc 301 cttcccctgg tgccagtctc actagcatgg cggcatctca gaatcatgat gctgacaatg 361 tccctaatct catggatgaa accaagactt tctcatcaac cgagagcctg cgacaactct 421 cccaacagct caatggtctt gtttgtgagt ctgcgacatg tgtcaatggg gagggccctg 481 catcgtctgc taacctgaag gatctggaga gccggtacca acagctagcg gtagccctgg 541 actccagcta tgtaacaaac aaacaactca atatcacgat agagaaattg aaacaacaga 601 accaagaaat tacggatcag ttggaagaag aaaagaaaga atgccaccaa aagcagggag 661 ccctaaggga gcagttacag gttcacattc agaccatagg gatcctcgta tcagagaaag 721 ctgagttaca gacagccctg gctcacactc agcatgctgc caggcagaaa gaaggagagt 781 ctgaagatct ggccagccgc ctgcagtatt cccggcggcg tgtgggagag ttggagcggg 841 ctctctctgc tgtctccacg cagcagaaga aggcagacag gtacaacaag gagttaacca 901 aagagagaga cgccctcagg ctggagttat acaagaacac ccaaagcaat gaggacctga 961 agcaagagaa atcagaattg gaagagaagc ttcgggtcct agtgactgag aaggctggca 1021 tgcagcttaa cttggaagaa ttgcaaaaga agttagagat gacggaactc ctgcttcaac 1081 agttttcaag ccggtgtgaa gcccctgatg ctaaccagca gttacagcag gccatggagg 1141 agcgggcaca gctggaagca cacctggggc aggtaatgga gtcggttaga caactacaaa 1201 tggagagaga taaatatgcg gagaatctca aaggagagag cgccatgtgg cggcagagga 1261 tgcagcagat gtcagagcag gtgcacacat tgagagagga gaaggaatgt agcatgagtc 1321 gggtacagga gctggagacg agcttggctg aactgaggaa ccagatggct gaacccccgc 1381 ccccagagcc cccagcaggg ccctccgagg tggagcagca gctacaagcg gaggctgagc 1441 acctgcggaa ggagctggag ggtctggcag gacagcttca agcccaggtg caagacaatg 1501 agggcttgag tcgcctgaac cgggagcagg aggagaggct gctggagctg gagcgggcgg 1561 ccgagctctg gggggagcag gcggaggcgc gcaggcaaat cctggagacc atgcagaacg 1621 accgcactac catcagccgc gcactctccc agaaccggga gctcaaggag cagctggctg 1681 agctgcagag cggatttgta aagctgacta atgagaacat ggagatcacc agcgcactgc 1741 agtcggagca gcacgtcaag agggagctgg gaaagaagct gggcgagctg caggagaagc 1801 tgagcgagct gaaggaaacg gtggagctga agagccaaga ggctcaaagt ctgcagcagc 1861 agcgagacca gtacctggga cacctgcagc agtatgtggc cgcctatcag cagctgacct 1921 ctgagaagga ggtgctgcat aatcagctac tgctgcagac ccagctcgtg gaccagctgc 1981 agcagcagga agctcagggc aaagcggtgg ccgagatggc ccgccaagag ttgcaggaaa 2041 cccaggagcg cctggaagct gccacccagc agaatcagca gctacgggcc cagttgagcc 2101 tcatggctca ccctggggaa ggagatggac tggaccggga ggaggaggag gatgaggagg 2161 aggaggagga ggaggcggtg gcagtacctc agcccatgcc aagcatcccg gaggacctgg 2221 agagccggga agccatggtg gcatttttca actcagctgt agccagtgcc gaggaggagc 2281 aggcaaggct acgtgggcag ctgaaggagc aaagggtgcg ctgccggcgc ctggctcacc 2341 tgctggcctc ggcccagaag gagcctgagg cagcagcccc agccccaggg accgggggtg 2401 attctgtgtg tggggagacc caccgggccc tgcagggggc catggagaag ctgcagagcc 2461 gctttatgga gctcatgcag gagaaggcag acctgaagga gagggtagag gaactggaac 2521 atcgctgcat ccagctttct ggagagacag acaccattgg agagtacatt gcactgtacc 2581 agagccagag ggcagtgctg aaggagcggc accgggagaa ggaggagtac atcagcaggc 2641 tggcccaaga caaggaggag atgaaggtga agctgctgga gctgcaggag ctggtcttac 2701 ggcttgtggg cgaccgcaac gagtggcatg gcagattcct ggcagctgcc cagaaccctg 2761 ctgatgagcc cacttcaggg gccccagccc cccaggaact tggggctgcc aaccagcagg 2821 gtgatctttg cgaggtgagc ctcgccggca gtgtggagcc tgcccaagga gaggccaggg 2881 agggttctcc ccgtgacaac cccactgcac agcagatcat gcagctgctt cgtgagatgc 2941 agaacccccg ggagcgccca ggcttgggca gcaacccctg cattcctttt ttttaccggg 3001 ctgacgagaa tgatgaggtg aagatcactg tcatctaccc aactttcttg tacaaagtgg 3061 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 3121 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta