Construct: ORF ccsbBroad304_10859
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014677.1_s304c1, BRDN0000408016
- Derived from:
- ccsbBroadEn_10859
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPX4 (2879)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10859
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2879 | GPX4 | glutathione peroxidase 4 | NM_001367832.1 | 40.5% | 16.4% | 1_303del |
| 2 | human | 2879 | GPX4 | glutathione peroxidase 4 | NM_002085.5 | 35% | 12.5% | 1_384del |
| 3 | human | 2879 | GPX4 | glutathione peroxidase 4 | NM_001039847.3 | 30.3% | 12.5% | 1_384del;500_521del;614_681del |
| 4 | human | 2879 | GPX4 | glutathione peroxidase 4 | NM_001039848.4 | 29.4% | 9% | 1_495del |
| 5 | mouse | 625249 | Gpx4 | glutathione peroxidase 4 | NM_008162.3 | 30.9% | 12.3% | (many diffs) |
| 6 | mouse | 625249 | Gpx4 | glutathione peroxidase 4 | NM_001037741.3 | 24.1% | 8.4% | (many diffs) |
| 7 | mouse | 625249 | Gpx4 | glutathione peroxidase 4 | NR_110342.1 | 20.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 273
- ORF length:
- 207
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt cagcaagatc tgcgtgaacg gggacgacgc ccacccgctg tggaagtgga 121 tgaagatcca acccaagggc aagggcatcc tgggaaatgc catcaagtgg aacttcacca 181 agttcctcat cgacaagaac ggctgcgtgg tgaagcgcta cggacccatg gaggagcccc 241 tggtgataga gaaggacctg ccccactatt tctacccaac tttcttgtac aaagtggttg 301 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 361 accggtggcg cgttaagtcg acaatcaacc tctggatta