Construct: ORF ccsbBroad304_10868
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015122.1_s304c1, BRDN0000411495
- Derived from:
- ccsbBroadEn_10868
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GUCY1A1 (2982)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10868
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | NM_001130687.2 | 22.2% | 19.9% | (many diffs) |
| 2 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | NM_000856.6 | 20.1% | 18% | (many diffs) |
| 3 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | NM_001130682.3 | 20.1% | 18% | (many diffs) |
| 4 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | NM_001130683.4 | 20.1% | 18% | (many diffs) |
| 5 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | NM_001130684.3 | 20.1% | 18% | (many diffs) |
| 6 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | NM_001256449.2 | 20.1% | 18% | (many diffs) |
| 7 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | XM_005262955.3 | 20.1% | 18% | (many diffs) |
| 8 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | XM_006714196.2 | 20.1% | 18% | (many diffs) |
| 9 | human | 2982 | GUCY1A1 | guanylate cyclase 1 soluble... | XM_006714197.2 | 20.1% | 18% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 495
- ORF length:
- 429
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt ctgcacgaag ctcaaggatc tcaagatcac aggagagtgt cctttctcct 121 tactggcacc aggtcaagtt cctaacgagt cttcagagga ggcagcagga agctcagaga 181 gctgcaaagc aaccgtgccc atctgtcaag acattcctga gaagaacata caagaaagtc 241 ttcctcaaag aaaaaccagt cggagccgag tctatcttca cactttggca gagagtattt 301 gcaaactgat tttcccagag tttgaacggc tgaatgttgc acttcagaga acattggcaa 361 agcacaaaat aaaagaaagc aggaaatctt tggaaagaga agactttgaa aaaacaattg 421 cagagcaagc agttgcagca ggtggagacc attggcgatg cctattgtgt agctggggga 481 ttacacaaag agagtgccca actttcttgt acaaagtggt tggtaagcct atccctaacc 541 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 601 cgacaatcaa cctctggatt a