Construct: ORF ccsbBroad304_10889
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016344.1_s304c1, BRDN0000408515
- Derived from:
- ccsbBroadEn_10889
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOXD12 (3238)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10889
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3238 | HOXD12 | homeobox D12 | NM_021193.4 | 74.9% | 70.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 720
- ORF length:
- 651
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtgtgagcgc agtctctaca gagcgggcta tgtgggctcg cttctgaatc 121 tgcagtcgcc agactctttc tacttctcca acctgaggcc gaatggcggc cagttggccg 181 cgcttccccc tatctcctac ccgcgcggcg cgctgccctg ggccgccacg cccgcctcct 241 gcgcccccgc gcagcctgcg ggcgccactg ccttcggcgg cttctcgcag ccctacctgg 301 ctggctccgg gcctctcggc ctgcagcccc caacagccaa agacggaccc gaagagcagg 361 ctaagttcta tgcgcccgaa gcggccgctg ggccagagga gcgcggtcgt acccggccgt 421 ccttcgcccc cgagtctagc ctggctcctg cagtggctgc tctcaaagcg gccaagtatg 481 actacgctgg tgtgggtcgt gccacgccgg gctccacgac cctgctccag ggggctccct 541 gcgcccctgg cttcaaggac gacaccaagg gcccgctcaa cttgaacatg acagtgcagg 601 cggcgggcgt tgcctcttgc ctgcgacctt cactgcccga cggtaaacgg tgcccatgct 661 ccccgggccg gtttgggccg ggatgggagg tggggttcaa gggagagtgt aaggggaggt 721 tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 781 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 841 ggatta