Construct: ORF ccsbBroad304_10972
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002133.1_s304c1, BRDN0000408018
- Derived from:
- ccsbBroadEn_10972
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRPM1 (4308)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10972
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4308 | TRPM1 | transient receptor potentia... | NM_001252030.2 | 99.7% | 99.2% | 95G>A |
| 2 | human | 4308 | TRPM1 | transient receptor potentia... | NM_002420.6 | 7.2% | 5.2% | (many diffs) |
| 3 | human | 4308 | TRPM1 | transient receptor potentia... | NM_001252024.2 | 7.1% | 5.1% | (many diffs) |
| 4 | human | 4308 | TRPM1 | transient receptor potentia... | NM_001252020.1 | 7% | 5.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 474
- ORF length:
- 408
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa agactctaac aggtgttgct gtggccagtt caccaaccag catatccccc 121 ctctgccaag tgcaacaccc agcaaaaatg aagaggaaaa caaacaggtg gagactcagc 181 ctgagaaatg gtctgttgcc aagcacaccc agagctaccc aacagattcc tatggagttc 241 ttgaattcca gggtggcgga tattccaata aagccatggt gagaaaggca ttcagacatg 301 gtgccactag gatcacagct ttcattggcg gccagtctcc cagccccaaa ctgcagatac 361 ctggtcttct tcatggctgt ggctcaatct tcctagatat ttcattgaaa aaccaagaga 421 tatatctgtg cacatggctt ttagccatga ggcttggaaa ctggacacca ctgtacccaa 481 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 541 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta