Construct: ORF ccsbBroad304_10973
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007636.1_s304c1, BRDN0000406832
- Derived from:
- ccsbBroadEn_10973
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MMP15 (4324)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10973
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4324 | MMP15 | matrix metallopeptidase 15 | NM_002428.4 | 84.2% | 84.1% | 1_315del;1825G>A |
| 2 | mouse | 17388 | Mmp15 | matrix metallopeptidase 15 | NM_008609.4 | 71.7% | 75.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1758
- ORF length:
- 1692
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa gcggccccgc tgtggggtgc cagaccagtt cggggtacga gtgaaagcca 121 acctgcggcg gcgtcggaag cgctacgccc tcaccgggag gaagtggaac aaccaccatc 181 tgacctttag catccagaac tacacggaga agttgggctg gtaccactcg atggaggcgg 241 tgcgcagggc cttccgcgtg tgggagcagg ccacgcccct ggtcttccag gaggtgccct 301 atgaggacat ccggctgcgg cgacagaagg aggccgacat catggtactc tttgcctctg 361 gcttccacgg cgacagctcg ccgtttgatg gcaccggtgg ctttctggcc cacgcctatt 421 tccctggccc cggcctaggc ggggacaccc attttgacgc agatgagccc tggaccttct 481 ccagcactga cctgcatgga aacaacctct tcctggtggc agtgcatgag ctgggccacg 541 cgctggggct ggagcactcc agcaacccca atgccatcat ggcgccgttc taccagtgga 601 aggacgttga caacttcaag ctgcccgagg acgatctccg tggcatccag cagctctacg 661 gtaccccaga cggtcagcca cagcctaccc agcctctccc cactgtgacg ccacggcggc 721 caggccggcc tgaccaccgg ccgccccggc ctccccagcc accaccccca ggtgggaagc 781 cagagcggcc cccaaagccg ggccccccag tccagccccg agccacagag cggcccgacc 841 agtatggccc caacatctgc gacggggact ttgacacagt ggccatgctt cgcggggaga 901 tgttcgtgtt caagggccgc tggttctggc gagtccggca caaccgcgtc ctggacaact 961 atcccatgcc catcgggcac ttctggcgtg gtctgcccgg tgacatcagt gctgcctacg 1021 agcgccaaga cggtcgtttt gtctttttca aaggtgaccg ctactggctc tttcgagaag 1081 cgaacctgga gcccggctac ccacagccgc tgaccagcta tggcctgggc atcccctatg 1141 accgcattga cacggccatc tggtgggagc ccacaggcca caccttcttc ttccaagagg 1201 acaggtactg gcgcttcaac gaggagacac agcgtggaga ccctgggtac cccaagccca 1261 tcagtgtctg gcaggggatc cctgcctccc ctaaaggggc cttcctgagc aatgacgcag 1321 cctacaccta cttctacaag ggcaccaaat actggaaatt cgacaatgag cgcctgcgga 1381 tggagcccgg ctaccccaag tccatcctgc gggacttcat gggctgccag gagcacgtgg 1441 agccaggccc ccgatggccc gacgtggccc ggccgccctt caacccccac gggggtgcag 1501 agcccggggc ggacagcgca gagggcgacg tgggggatgg ggatggggac tttggggccg 1561 gggtcaacaa ggacaggggc agccgcgtgg tggtgcagat ggaggaggtg gcacggacgg 1621 tgaacgtggt gatggtgctg gtgccactgc tgctgctgct ctgcgtcctg ggcctcacct 1681 acgcgctggt gcagatgcag cgcaagggtg cgccacgtgt cctgctttac tgcaagcgct 1741 cgctgcagga gtgggtctgc ccaactttct tgtacaaagt ggttggtaag cctatcccta 1801 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 1861 agtcgacaat caacctctgg atta