Construct: ORF ccsbBroad304_11097
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011293.1_s304c1, BRDN0000404596
- Derived from:
- ccsbBroadEn_11097
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RFC1 (5981)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11097
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5981 | RFC1 | replication factor C subunit 1 | NM_001204747.2 | 99.9% | 100% | 2544A>G |
| 2 | human | 5981 | RFC1 | replication factor C subunit 1 | NM_002913.5 | 99.8% | 99.9% | 1884_1885insGCA;2541A>G |
| 3 | human | 5981 | RFC1 | replication factor C subunit 1 | NM_001363496.2 | 97.7% | 97.7% | 640_641ins78;2466A>G |
| 4 | human | 5981 | RFC1 | replication factor C subunit 1 | NM_001363495.2 | 97.6% | 97.6% | 640_641ins78;1806_1807insGCA;2463A>G |
| 5 | human | 5981 | RFC1 | replication factor C subunit 1 | XM_011513731.1 | 85.1% | 85.1% | 1486_1487ins510;2034A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3510
- ORF length:
- 3444
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cattcggaaa ttctttggag taataccaag tggaaagaaa cttgtaagtg 121 aaacagtaaa gaagaatgag aaaacaaagt ctgatgaaga aactttaaaa gcaaagaaag 181 gaataaagga aatcaaggta aatagctccc gtaaagagga tgacttcaaa caaaagcaac 241 caagcaagaa aaagaggatc atctatgatt cagattcaga gtcagaggag acgttgcagg 301 taaaaaatgc caaaaagcca ccagaaaaac tgccagtatc ttctaaacct ggtaaaattt 361 cacggcagga tcctgttaca tacatttcag aaacagatga agaagatgac tttatgtgta 421 agaaggcggc ctctaaatca aaagagaatg gaagatctac aaatagtcat cttggaacat 481 caaacatgaa aaagaatgaa gaaaacacta agaccaagaa taagccttta tcaccaataa 541 aacttacacc cacatcagta cttgattatt ttggaactgg aagtgtccaa agatctaata 601 agaagatggt ggcaagcaaa agaaaagagc tttcacaaaa tacagatgag tctggattaa 661 atgatgaagc catcgccaag caattacagc ttgatgaaga tgcggagctg gagaggcagt 721 tgcatgaaga tgaagagttt gccagaacat tagccatgtt ggatgaagaa cccaagacca 781 aaaaggctcg aaaggacaca gaagcgggag aaacgttttc atctgtccaa gccaatttaa 841 gtaaagcaga aaaacataaa tatcctcata aagtaaaaac agcacaagtt tcagatgaaa 901 gaaagagcta cagtcctagg aagcaaagta aatatgaaag ttcaaaagaa tctcagcaac 961 attccaagtc atcagctgac aaaataggag aagtctcttc tcccaaggcc agttctaagc 1021 tggcaattat gaaaagaaaa gaagagagct cttataaaga aatagagcct gtggcctcaa 1081 aaagaaaaga aaatgccatt aaattgaaag gagagacaaa aactcctaag aaaaccaaaa 1141 gttctccagc taaaaaagag tctgtaagtc ctgaagattc tgaaaagaaa cgcactaatt 1201 atcaagctta tcgaagctac ttaaatcgag aaggtcccaa ggctctgggc tccaaagaaa 1261 taccaaaggg agctgaaaat tgcttggaag gccttatatt tgtaatcaca ggcgtgctgg 1321 agtctattga acgagatgag gccaagtctc taattgaacg ttatggggga aaagtaacag 1381 gaaatgtcag caagaaaaca aattatcttg tcatgggtcg tgatagtgga cagtccaaga 1441 gtgataaggc cgcagccttg gggacaaaaa ttattgatga agatggcctg ttgaatctga 1501 ttcggactat gccaggcaag aaatccaagt atgaaatagc agttgaaact gagatgaaga 1561 aagagtccaa actggagaga acaccccaaa aaaatgtcca aggaaaaaga aaaattagtc 1621 catctaaaaa ggaatcagaa tctaaaaaga gcaggccgac ttccaaaagg gacagtttgg 1681 caaagacaat aaaaaaggaa acagatgtgt tttggaaaag cctggatttc aaggagcagg 1741 tggctgagga gacaagtggt gacagcaagg ctaggaattt ggctgatgac agcagtgaaa 1801 acaaagtgga aaatttgctc tgggtggata aatataagcc aacctcgctc aagaccataa 1861 ttggacagca aggtgaccag agctgtgcca acaaactcct acgctggctc cgaaactggc 1921 aaaagagttc ttccgaagat aaaaaacacg cagcaaagtt tggtaaattt tccggcaaag 1981 atgatggctc tagttttaaa gcagcgttgc tgtcaggccc tcctggtgtt ggcaaaacca 2041 ccacagcttc cctggtgtgt caggagttgg gatacagcta cgtggaactg aatgcaagtg 2101 acacccggag taagagcagt ttgaaggcga ttgttgctga gtcactgaac aataccagca 2161 tcaaaggctt ttattcaaat ggagcagcct cttcagtaag cacgaaacat gctctcatca 2221 tggatgaagt agatggcatg gcaggcaatg aggatagggg aggaattcag gaattaattg 2281 gcctgataaa acatactaaa attcccatta tttgtatgtg caatgataga aatcatccca 2341 agattcgctc tctggttcat tattgttttg atcttcgttt tcaaagacct cgggttgaac 2401 agattaaggg tgctatgatg tctattgcat ttaaagaagg tttaaagatt ccccctccag 2461 ctatgaatga aataattttg ggagccaatc aagatatcag acaggtttta cataatctga 2521 gtatgtggtg tgcacgaagt aaagcattaa cctatgacca ggccaaagct gattctcaca 2581 gagccaaaaa ggatatcaaa atgggcccgt ttgatgttgc ccggaaagtg tttgcagctg 2641 gagaggagac tgctcacatg tcacttgtgg acaagtcaga tctctttttt catgattatt 2701 caatagcacc cctcttcgtc caggaaaatt acatacacgt gaagcctgta gcagcagggg 2761 gtgacatgaa aaagcacctg atgcttttaa gcagagcagc agacagcata tgcgatggtg 2821 acctagtgga cagccagatc cggagtaagc aaaactggag tcttctgcct gcgcaggcca 2881 tttatgccag tgttcttcct ggagagttga tgagggggta catgacccag tttcccacct 2941 tcccaagctg gctggggaag cactcgtcta caggcaaaca tgatcgtatt gttcaggacc 3001 tggccttgca tatgagtctc agaacttact ccagcaaaag gactgtaaac atggattatc 3061 tgtcgcttct aagggatgca cttgtacagc ccttgacctc acaaggagta gacggagtac 3121 aggatgttgt tgcacttatg gacacatatt atttgatgaa agaagacttt gagaatatca 3181 tggaaatcag cagctggggt ggcaaaccta gtcccttttc aaagctggat cccaaggtga 3241 aagcagcctt cacaagagct tacaataagg aagcccacct tactccatac tcacttcaag 3301 ctataaaggc atctagacac agcacaagcc catccctgga ttcggaatac aatgaagaat 3361 taaatgaaga tgactctcaa tctgatgaga aagaccaaga tgctatagaa actgatgcca 3421 tgatcaagaa aaagacaaaa tcttcaaagc cttcaaaacc agaaaaagat aaggagccca 3481 gaaaaggaaa aggaaaaagt tcgaagaaat gcccaacttt cttgtacaaa gtggttggta 3541 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 3601 ggtggcgcgt taagtcgaca atcaacctct ggatta