Construct: ORF ccsbBroad304_11100

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007118.1_s304c1, BRDN0000399326
Derived from:
ccsbBroadEn_11100
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RGS3 (5998)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11100
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5998 RGS3 regulator of G protein sign... NM_001276262.2 87.3% 86.9% 0_1ins72;35A>G
2 human 5998 RGS3 regulator of G protein sign... NM_134427.2 87.3% 86.9% 0_1ins72;35A>G
3 human 5998 RGS3 regulator of G protein sign... NM_144489.3 61.6% 61.4% 1_357del;464A>G
4 human 5998 RGS3 regulator of G protein sign... NM_001276260.2 35.5% 33.3% (many diffs)
5 human 5998 RGS3 regulator of G protein sign... NM_001282922.2 35.5% 33.3% (many diffs)
6 human 5998 RGS3 regulator of G protein sign... NM_001351526.2 35.5% 33.3% (many diffs)
7 human 5998 RGS3 regulator of G protein sign... NM_001322215.2 34.3% 32.2% (many diffs)
8 human 5998 RGS3 regulator of G protein sign... NM_001276261.1 31.4% 29.2% (many diffs)
9 human 5998 RGS3 regulator of G protein sign... NM_130795.4 20.1% 18.8% (many diffs)
10 human 5998 RGS3 regulator of G protein sign... NM_001282923.2 17% 15.9% (many diffs)
11 human 5998 RGS3 regulator of G protein sign... NM_144488.6 15.4% 14.4% (many diffs)
12 mouse 50780 Rgs3 regulator of G-protein sign... XM_017320308.1 87.3% 92.7% (many diffs)
13 mouse 50780 Rgs3 regulator of G-protein sign... XM_006538072.3 75.6% 80.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
642
ORF length:
576
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct ccgaggcatg tacctcactc gcaacgggaa cctgcagagg cgacacacga
121tgaaggaagc caaggacatg aagaacaagc tggggatctt cagacggcgg agtgagtccc
181ctggagcccc tcccgcgggc aaggcagaca aaatgatgaa gtcattcaag cccacctcag
241aggaagccct caagtggggc gagtccttgg agaagctgct ggttcacaaa tacgggttag
301cagtgttcca agccttcctt cgcactgagt tcagtgagga gaatctggag ttctggttgg
361cttgtgagga cttcaagaag gtcaagtcac agtccaagat ggcatccaag gccaagaaga
421tctttgctga atacatcgcg atccaggcat gcaaggaggt caacctggac tcctacacgc
481gggagcacac caaggacaac ctgcagagcg tcacgcgggg ctgcttcgac ctggcacaga
541agcgcatctt cgggctcatg gaaaaggact cgtaccctcg ctttctccgt tctgacctct
601acctggacct tattaaccag aagaagatga gtcccccgct ttacccaact ttcttgtaca
661aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
721ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)