Construct: ORF ccsbBroad304_11140
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002497.1_s304c1, BRDN0000409435
- Derived from:
- ccsbBroadEn_11140
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC18A1 (6570)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11140
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6570 | SLC18A1 | solute carrier family 18 me... | NM_001142325.1 | 81% | 81.1% | (many diffs) |
| 2 | human | 6570 | SLC18A1 | solute carrier family 18 me... | NM_001135691.2 | 72.8% | 72.9% | (many diffs) |
| 3 | human | 6570 | SLC18A1 | solute carrier family 18 me... | NM_003053.4 | 72.8% | 72.9% | (many diffs) |
| 4 | human | 6570 | SLC18A1 | solute carrier family 18 me... | XM_011544625.1 | 67.5% | 67.4% | (many diffs) |
| 5 | human | 6570 | SLC18A1 | solute carrier family 18 me... | NM_001142324.2 | 66.7% | 66.8% | (many diffs) |
| 6 | human | 6570 | SLC18A1 | solute carrier family 18 me... | XM_011544626.1 | 66.7% | 66.8% | (many diffs) |
| 7 | mouse | 110877 | Slc18a1 | solute carrier family 18 (v... | XM_011242262.2 | 53.8% | 54.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1221
- ORF length:
- 1155
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct ccggaccatt ctggatgctc cccagcggtt gctgaaggag gggagagcgt 121 cccggcagct ggtgctggtg gtggtattcg tcgctttgct cctggacaac atgctgttta 181 ctgtggtggt gccaattgtg cccaccttcc tatatgacat ggagttcaaa gaagtcaact 241 cttctctgca cctcggccat gccggaagtt ccccacatgc cctcgcctct cctgcctttt 301 ccaccatctt ctccttcttc aacaacaaca ccgtggctgt tgaagaaagc gtacctagtg 361 gaatagcatg gatgaatgac actgccagca ccatcccacc tccagccact gaagccatct 421 cagctcataa aaacaactgc ttgcaaggca caggtttctt ggaggaagag attacccggg 481 tcggggttct gtttgcttca aaggctgtga tgcaacttct ggtcaaccca ttcgtgggcc 541 ctctcaccaa caggattgga tatcatatcc ccatgtttgc tggctttgtt atcatgtttc 601 tctccacagt tatgtttgct ttttctggga cctatactct actctttgtg gcccgaaccc 661 ttcaaggcat tggatcttca ttttcatctg ttgcaggtct tggaatgctg gccagtgtct 721 acactgatga ccatgagaga ggacgagcca tgggaactgc tctggggggc ctggccttgg 781 ggttgctggt gggagctccc tttggaagtg taatgtacga gtttgttggg aagtctgcac 841 ccttcctcat cctggccttc ctggcactac tggatggagc actccagctt tgcatcctac 901 agccttccaa agtctctcct gagagtgcca aggggactcc cctctttatg cttctcaaag 961 acccttacat cctggtggct gcagggtcca tctgctttgc caacatgggg gtggccatcc 1021 tggagcccac actgcccatc tggatgatgc agaccatgtg ctcccccaag tggcagctgg 1081 gtctagcttt cttgcctgcc agtgtgtcct acctcattgg caccaacctc tttggtgtgt 1141 tggccaacaa gatgggtcgg tggctgtgtt ccctaatcgg gatgctggta gtaggtacca 1201 gcttgctctg tctaccaagt tacccaactt tcttgtacaa agtggttggt aagcctatcc 1261 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1321 ttaagtcgac aatcaacctc tggatta