Construct: ORF ccsbBroad304_11142

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015879.1_s304c1, BRDN0000407998
Derived from:
ccsbBroadEn_11142
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SLC22A2 (6582)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11142
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6582 SLC22A2 solute carrier family 22 me... NM_003058.4 42.3% 39.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
792
ORF length:
726
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc caccaccgtg gacgatgtcc tggagcatgg aggggagttt cactttttcc
121agaagcaaat gtttttcctc ttggctctgc tctcggctac cttcgcgccc atctacgtgg
181gcatcgtctt cctgggcttc acccctgacc accgctgccg gagccccgga gtggccgagc
241tgagtctgcg ctgcggctgg agtcctgcag aggaactgaa ctacacggtg ccgggcccag
301gacctgcggg cgaagcctcc ccaagacagt gtaggcgcta cgaggtggac tggaaccaga
361gcaccttcga ctgcgtggac cccctggcca gcctggacac caacaggagc cgcctgccac
421tgggcccctg ccgggacggc tgggtgtacg agacgcctgg ctcgtccatc gtcaccgagt
481ttaacctggt atgtgccaac tcctggatgt tggacctatt ccagtcatca gtgaatgtag
541gattctttat tggctctatg agtatcggct acatagcaga caggtttggc cgtaagctct
601gcctcctaac tacagtcctc ataaatgctg cagctggagt tctcatggcc atttccccaa
661cctatacgtg gatgttaatt tttcgcttaa tccaaggact ggtcagcaaa gcaggctggt
721taataggcta catcctgagt aagaatgttt gtgcttgcaa ctgtgagaac aaagcaacct
781cgctgccaaa atacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
841tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
901caatcaacct ctggatta

Download FASTA (ORF) (Full)