Construct: ORF ccsbBroad304_11186
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003823.1_s304c1, BRDN0000400571
- Derived from:
- ccsbBroadEn_11186
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TGFBR2 (7048)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11186
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7048 | TGFBR2 | transforming growth factor ... | NM_003242.6 | 58.8% | 22.5% | 373_374insA;1003_1701del |
| 2 | human | 7048 | TGFBR2 | transforming growth factor ... | NM_001024847.2 | 56.3% | 21.4% | 95_169del;448_449insA;1078_1776del |
| 3 | human | 7048 | TGFBR2 | transforming growth factor ... | XM_011534043.2 | 54.7% | 16.9% | (many diffs) |
| 4 | human | 7048 | TGFBR2 | transforming growth factor ... | XM_011534045.3 | 52.7% | 16.4% | 0_1ins105;268_269insA;898_1596del |
| 5 | human | 7048 | TGFBR2 | transforming growth factor ... | XM_017007106.1 | 52.7% | 16.4% | 0_1ins105;268_269insA;898_1596del |
| 6 | mouse | 21813 | Tgfbr2 | transforming growth factor,... | NM_029575.3 | 50.3% | 19.7% | (many diffs) |
| 7 | mouse | 21813 | Tgfbr2 | transforming growth factor,... | NM_009371.3 | 48.7% | 18.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 456
- ORF length:
- 390
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg tcgggggctg ctcaggggcc tgtggccgct gcacatcgtc ctgtggacgc 121 gtatcgccag cacgatccca ccgcacgttc agaagtcggt taataacgac atgatagtca 181 ctgacaacaa cggtgcagtc aagtttccac aactgtgtaa attttgtgat gtgagatttt 241 ccacctgtga caaccagaaa tcctgcatga gcaactgcag catcacctcc atctgtgaga 301 agccacagga agtctgtgtg gctgtatgga gaaagaatga cgagaacata acactagaga 361 cagtttgcca tgaccccaag ctcccctacc atgactttat tcTGGAAGAT GCTGCTTCTC 421 CAAAGTGCAT TATGAAGGAA AAAAAAAAAG CCTGGTGAGA CTTTCTTCAT GTGTTCCTGT 481 AGCTCTGATG AGTGCAATGA CAACATCATC TTCTCAGAAG AATATAACAC CAGCAATCCT 541 GACTTGTTGC TAGTCATATT TCAAGTGACA GGCATCAGCC TCCTGCCACC ACTGGGAGTT 601 GCCATATCTG TCATCATCAT CTTCTACTGC TACCGCGTTA ACCGGCAGCA GAAGCTGAGT 661 TCAACCTGGG AAACCGGCAA GACGCGGAAG CTCATGGAGT TCAGCGAGCA CTGTGCCATC 721 ATCCTGGAAG ATGACCGCTC TGACATCAGC TCCACGTGTG CCAACAACAT CAACCACAAC 781 ACAGAGCTGC TGCCCATTGA GCTGGACACC CTGGTGGGGA AAGGTCGCTT TGCTGAGGTC 841 TATAAGGCCA AGCTGAAGCA GAACACTTCA GAGCAGTTTG AGACAGTGGC AGTCAAGATC 901 TTTCCCTATG AGGAGTATGC CTCTTGGAAG ACAGAGAAGG ACATCTTCTC AGACATCAAT 961 CTGAAGCATG AGAACATACT CCAGTTCCTG ACGGCTGAGG AGCGGAAGAC GGAGTTGGGG 1021 AAACAATACT GGCTGATCAC CGCCTTCCAC GCCAAGGGCA ACCTACAGTA CCCAACTTTC 1081 TTGTACaaag tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag 1141 taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta