Construct: ORF ccsbBroad304_11248
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006465.1_s304c1, BRDN0000402570
- Derived from:
- ccsbBroadEn_11248
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPM2A (7957)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11248
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | NM_001368129.2 | 49.7% | 49.7% | 1_267del |
| 2 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | NM_001368132.1 | 49.7% | 49.7% | 1_267del |
| 3 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | XM_017011301.1 | 49.7% | 49.7% | 1_267del |
| 4 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | XM_017011302.1 | 49.7% | 49.7% | 1_267del |
| 5 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | NM_001360064.2 | 45.5% | 45.5% | 1_315del |
| 6 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | NM_001360071.2 | 45.5% | 45.5% | 1_315del |
| 7 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | NM_001368131.1 | 45.5% | 45.5% | 1_315del |
| 8 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | XM_011536116.1 | 45.5% | 45.5% | 1_315del |
| 9 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | XM_024446551.1 | 45.5% | 45.5% | 1_315del |
| 10 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | NM_005670.4 | 26.5% | 26.5% | 1_729del |
| 11 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | NM_001360057.2 | 24.6% | 1.6% | 1_487del;672_673ins79 |
| 12 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | NM_001018041.2 | 21.6% | 20.8% | (many diffs) |
| 13 | human | 7957 | EPM2A | EPM2A glucan phosphatase, l... | NR_153398.2 | 9.8% | 1_302del;567_2680del | |
| 14 | mouse | 13853 | Epm2a | epilepsy, progressive myocl... | NM_010146.2 | 21.7% | 23% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 330
- ORF length:
- 264
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct gccccaggcg gtgtgcctgc tgcatgcgct gctggagaag ggacacatcg 121 tgtacgtgca ctgcaacgct ggggtgggcc gctccaccgc ggctgtctgc ggctggctcc 181 agtatgtgat gggctggaat ctgaggaagg tgcagtattt cctcatggcc aagaggccgg 241 ctgtctacat tgacgaagag gccttggccc gggcacaaga agattttttc cagaaatttg 301 ggaaggttcg ttcttctgtg tgtagcctgt acccaacttt cttgtacaaa gtggttggta 361 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 421 ggtggcgcgt taagtcgaca atcaacctct ggatta