Construct: ORF ccsbBroad304_11293
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007922.1_s304c1, BRDN0000407339
- Derived from:
- ccsbBroadEn_11293
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PEA15 (8682)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11293
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8682 | PEA15 | proliferation and apoptosis... | NR_123724.2 | 9.8% | 1_1489del;1712_2246del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 288
- ORF length:
- 222
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgta tattaaaact gcactgccat gtctgccctt ttttgtggtg tctagcatta 121 acttattgtc taggccagag cgggggtggg aggggaatgc cacagtgaag ggagtggcag 181 aatcaaattg ctacatagtc caaacaaaaa agaaggcttt ttcaaaaaac attaaattca 241 catgcagtct cagagactat ttagacaaag ttcaagttag gagcttttac ccaactttct 301 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 361 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta