Construct: ORF ccsbBroad304_11371

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014192.1_s304c1, BRDN0000408749
Derived from:
ccsbBroadEn_11371
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ROCK2 (9475)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11371
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9475 ROCK2 Rho associated coiled-coil ... NM_001321643.2 53.9% 53.9% 1_1797del
2 human 9475 ROCK2 Rho associated coiled-coil ... XM_011510417.2 52.7% 52.7% 1_1797del;3906_3993delinsC
3 human 9475 ROCK2 Rho associated coiled-coil ... XM_017005379.2 52.7% 52.7% 1_1797del;3906_3993delinsC
4 human 9475 ROCK2 Rho associated coiled-coil ... NM_004850.5 50.6% 50.6% 1_2055del
5 human 9475 ROCK2 Rho associated coiled-coil ... XM_017005378.2 50% 50% 1_2055del;4164_4212delinsC
6 human 9475 ROCK2 Rho associated coiled-coil ... XM_005246190.3 49.5% 49.5% 1_2055del;4164_4251delinsC
7 mouse 19878 Rock2 Rho-associated coiled-coil ... XM_006515019.3 50% 53.8% (many diffs)
8 mouse 19878 Rock2 Rho-associated coiled-coil ... XM_006515020.3 50% 53.8% (many diffs)
9 mouse 19878 Rock2 Rho-associated coiled-coil ... NM_009072.2 45.7% 49.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2178
ORF length:
2109
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggaaatagat atgacatacc aactaaaagt tatacagcag agcctagaac
121aagaagaagc tgaacataag gccacaaagg cacgactagc agataaaaat aagatctatg
181agtccatcga agaagccaaa tcagaagcca tgaaagaaat ggagaagaag ctcttggagg
241aaagaacttt aaaacagaaa gtggagaacc tattgctaga agctgagaaa agatgttctc
301tattagactg tgacctcaaa cagtcacagc agaaaataaa tgagctcctt aaacagaaag
361atgtgctaaa tgaggatgtt agaaacctga cattaaaaat agagcaagaa actcagaagc
421gctgccttac acaaaatgac ctgaagatgc aaacacaaca ggttaacaca ctaaaaatgt
481cagaaaagca gttaaagcaa gaaaataacc atctcatgga aatgaaaatg aacttggaaa
541aacaaaatgc tgaacttcga aaagaacgtc aggatgcaga tgggcaaatg aaagagctcc
601aggatcagct cgaagcagaa cagtatttct caacccttta taaaacacaa gttagggagc
661ttaaagaaga atgtgaagaa aagaccaaac ttggtaaaga attgcagcag aagaaacagg
721aattacagga tgaacgggac tctttggctg cccaactgga gatcaccttg accaaagcag
781attctgagca actggctcgt tcaattgctg aagaacaata ttctgatttg gaaaaagaga
841agatcatgaa agagctggag atcaaagaga tgatggctag acacaaacag gaacttacgg
901aaaaagatgc tacaattgct tctcttgagg aaactaatag gacactaact agtgatgttg
961ccaatcttgc aaatgagaaa gaagaattaa ataacaaatt gaaagatgtt caagagcaac
1021tgtcaagatt gaaagatgaa gaaataagcg cagcagctat taaagcacag tttgagaagc
1081agctattaac agaaagaaca ctcaaaactc aagctgtgaa taagttggct gagatcatga
1141atcgaaaaga acctgtcaag cgtggtaatg acacagatgt gcggagaaaa gagaaggaga
1201atagaaagct acatatggag cttaaatctg aacgtgagaa attgacccag cagatgatca
1261agtatcagaa agaactgaat gaaatgcagg cacaaatagc tgaagagagc cagattcgaa
1321ttgaactgca gatgacattg gacagtaaag acagtgacat tgagcagctg cggtcacaac
1381tccaagcctt gcatattggt ctggatagtt ccagtatagg cagtggacca ggggatgctg
1441aggcagatga tgggtttcca gaatcaagat tagaaggatg gctttcattg cctgtacgaa
1501acaacactaa gaaatttgga tgggttaaaa agtatgtgat tgtaagcagt aagaagattc
1561ttttctatga cagtgaacaa gataaagaac aatccaatcc ttacatggtt ttagatatag
1621acaagttatt tcatgtccga ccagttacac agacagatgt gtatagagca gatgctaaag
1681aaattccaag gatattccag attctgtatg ccaatgaagg agaaagtaag aaggaacaag
1741aatttccagt ggagccagtt ggagaaaaat ctaattatat ttgccacaag ggacatgagt
1801ttattcctac tctttatcat ttcccaacca actgtgaggc ttgtatgaag cccctgtggc
1861acatgtttaa gcctcctcct gctttggagt gccgccgttg ccatattaag tgtcataaag
1921atcatatgga caaaaaggag gagattatag caccttgcaa agtatattat gatatttcaa
1981cggcaaagaa tctgttatta ctagcaaatt ctacagaaga gcagcagaag tgggttagtc
2041ggttggtgaa aaagatacct aaaaagcccc cagctccaga cccttttgcc cgatcatctc
2101ctagaacttc aatgaagata cagcaaaacc agtctattag acggccaagt cgacagcttg
2161ccccaaacaa acctagcttg ccaactttct tgtacaaagt ggttggtaag cctatcccta
2221accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
2281agtcgacaat caacctctgg atta

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