Construct: ORF ccsbBroad304_11375
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014975.1_s304c1, BRDN0000408841
- Derived from:
- ccsbBroadEn_11375
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSMF1 (9491)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11375
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9491 | PSMF1 | proteasome inhibitor subunit 1 | NM_001323409.2 | 37.8% | 36% | 107T>G;282_364del;381_783del |
| 2 | human | 9491 | PSMF1 | proteasome inhibitor subunit 1 | NM_001323408.2 | 37.5% | 35.7% | 107T>G;282_364del;381_789del |
| 3 | human | 9491 | PSMF1 | proteasome inhibitor subunit 1 | NM_001323410.2 | 37.5% | 35.7% | 107T>G;282_364del;381_789del |
| 4 | human | 9491 | PSMF1 | proteasome inhibitor subunit 1 | NM_006814.5 | 36.4% | 34.6% | 107T>G;282_364del;381_813del |
| 5 | human | 9491 | PSMF1 | proteasome inhibitor subunit 1 | NM_178578.3 | 36.4% | 34.6% | 107T>G;282_364del;381_813del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 366
- ORF length:
- 297
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgggcctg gaggtactgt tcgcatcggc agcgccggcc atcacctgca 121 ggcaggacgc gctcgtctgc ttcttgcatt gggaagtggt gacacacggt tactgcggct 181 tgggtgtcgg tgaccagccg ggtcccaatg ataagaagtc agaactgctg ccagctgggt 241 ggaacaacaa taaagacctg tatgtcctcc ggtatgagta taaggatggg tccagaaagc 301 tccttgtgaa agccatcacc gtggagagca gcatgatcct caatgtgctg gacctacaag 361 aacagttgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 421 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 481 acctctggat ta