Transcript: Human NM_001323410.2

Homo sapiens proteasome inhibitor subunit 1 (PSMF1), transcript variant 6, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
PSMF1 (9491)
Length:
6972
CDS:
173..964

Additional Resources:

NCBI RefSeq record:
NM_001323410.2
NBCI Gene record:
PSMF1 (9491)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001323410.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000221081 CCCTGAACTTGGATGATTATA pLKO.1 486 CDS 100% 15.000 10.500 N PSMF1 n/a
2 TRCN0000333486 CCCTGAACTTGGATGATTATA pLKO_005 486 CDS 100% 15.000 10.500 N PSMF1 n/a
3 TRCN0000221078 GCTGGGTGGAACAACAATAAA pLKO.1 338 CDS 100% 15.000 10.500 N PSMF1 n/a
4 TRCN0000333546 GCTGGGTGGAACAACAATAAA pLKO_005 338 CDS 100% 15.000 10.500 N PSMF1 n/a
5 TRCN0000221079 GTCTGGAATCATCACACCTAT pLKO.1 577 CDS 100% 4.950 3.465 N PSMF1 n/a
6 TRCN0000333487 GTCTGGAATCATCACACCTAT pLKO_005 577 CDS 100% 4.950 3.465 N PSMF1 n/a
7 TRCN0000221080 GCACTTATTGACCCTTCCTCA pLKO.1 830 CDS 100% 2.640 1.848 N PSMF1 n/a
8 TRCN0000333547 GCACTTATTGACCCTTCCTCA pLKO_005 830 CDS 100% 2.640 1.848 N PSMF1 n/a
9 TRCN0000066617 GCAGACTTGACCCTGAACTTA pLKO.1 476 CDS 100% 5.625 3.938 N Psmf1 n/a
10 TRCN0000325579 GCAGACTTGACCCTGAACTTA pLKO_005 476 CDS 100% 5.625 3.938 N Psmf1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001323410.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07428 pDONR223 100% 95.1% 93% None (many diffs) n/a
2 ccsbBroad304_07428 pLX_304 0% 95.1% 93% V5 (many diffs) n/a
3 TRCN0000468513 TACATAGCAGATTGTGTGAAATTA pLX_317 41.2% 95.1% 93% V5 (many diffs) n/a
4 ccsbBroadEn_11374 pDONR223 100% 65.4% 50.3% None (many diffs) n/a
5 ccsbBroad304_11374 pLX_304 0% 65.4% 50.3% V5 (many diffs) n/a
6 TRCN0000470016 CCGTGTAAGATCCGAATACCGCAA pLX_317 62.8% 65.4% 50.3% V5 (many diffs) n/a
7 ccsbBroadEn_11375 pDONR223 100% 37.5% 35.7% None 107T>G;282_364del;381_789del n/a
8 ccsbBroad304_11375 pLX_304 0% 37.5% 35.7% V5 107T>G;282_364del;381_789del n/a
9 TRCN0000479575 AACCGCAAAATTTTAGCAGATCAT pLX_317 100% 37.5% 35.7% V5 107T>G;282_364del;381_789del n/a
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