Construct: ORF ccsbBroad304_11456
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009753.1_s304c1, BRDN0000406843
- Derived from:
- ccsbBroadEn_11456
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OPTN (10133)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11456
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10133 | OPTN | optineurin | NM_001008211.1 | 98.9% | 98.9% | 627_644del |
| 2 | human | 10133 | OPTN | optineurin | NM_001008212.2 | 98.9% | 98.9% | 627_644del |
| 3 | human | 10133 | OPTN | optineurin | NM_001008213.1 | 98.9% | 98.9% | 627_644del |
| 4 | human | 10133 | OPTN | optineurin | NM_021980.4 | 98.9% | 98.9% | 627_644del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1779
- ORF length:
- 1713
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc ccatcaacct ctcagctgcc tcactgaaaa ggaggacagc cccagtgaaa 121 gcacaggaaa tggacccccc cacctggccc acccaaacct ggacacgttt accccggagg 181 agctgctgca gcagatgaaa gagctcctga ccgagaacca ccagctgaaa gaagccatga 241 agctaaataa tcaagccatg aaagggagat ttgaggagct ttcggcctgg acagagaaac 301 agaaggaaga acgccagttt tttgagatac agagcaaaga agcaaaagag cgtctaatgg 361 ccttgagtca tgagaatgag aaattgaagg aagagcttgg aaaactaaaa gggaaatcag 421 aaaggtcatc tgaggacccc actgatgact ccaggcttcc cagggccgaa gcggagcagg 481 aaaaggacca gctcaggacc caggtggtga ggctacaagc agagaaggca gacctgttgg 541 gcatcgtgtc tgaactgcag ctcaagctga actccagcgg ctcctcagaa gattcctttg 601 ttgaaattag gatggctgaa ggagaagcag aagggtcagt aaaagaaatc aagcatagtc 661 ctgggcccac gagaacagtc tccactggca cgagcagatc tgcagatggg gccaagaatt 721 acttcgaaca tgaggagtta actgtgagcc agctcctgct gtgcctaagg gaagggaatc 781 agaaggtgga gagacttgaa gttgcactca aggaggccaa agaaagagtt tcagattttg 841 aaaagaaaac aagtaatcgt tctgagattg aaacccagac agaggggagc acagagaaag 901 agaatgatga agagaaaggc ccggagactg ttggaagcga agtggaagca ctgaacctcc 961 aggtgacatc tctgtttaag gagcttcaag aggctcatac aaaactcagc gaagctgagc 1021 taatgaagaa gagacttcaa gaaaagtgtc aggcccttga aaggaaaaat tctgcaattc 1081 catcagagtt gaatgaaaag caagagcttg tttatactaa caaaaagtta gagctacaag 1141 tggaaagcat gctatcagaa atcaaaatgg aacaggctaa aacagaggat gaaaagtcca 1201 aattaactgt gctacagatg acacacaaca agcttcttca agaacataat aatgcattga 1261 aaacaattga ggaactaaca agaaaagagt cagaaaaagt ggacagggca gtgctgaagg 1321 aactgagtga aaaactggaa ctggcagaga aggctctggc ttccaaacag ctgcaaatgg 1381 atgaaatgaa gcaaaccatt gccaagcagg aagaggacct ggaaaccatg accatcctca 1441 gggctcagat ggaagtttac tgttctgatt ttcatgctga aagagcagcg agagagaaaa 1501 ttcatgagga aaaggagcaa ctggcattgc agctggcagt tctgctgaaa gagaatgatg 1561 ctttcgaaga cggaggcagg cagtccttga tggagatgca gagtcgtcat ggggcgagaa 1621 caagtgactc tgaccagcag gcttaccttg ttcaaagagg agctgaggac agggactggc 1681 ggcaacagcg gaatattccg attcattcct gccccaagtg tggagaggtt ctgcctgaca 1741 tagacacgtt acagattcac gtgatggatt gcatcattta cccaactttc ttgtacaaag 1801 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1861 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta