Construct: ORF ccsbBroad304_11494
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007383.1_s304c1, BRDN0000404536
- Derived from:
- ccsbBroadEn_11494
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAPGEF3 (10411)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11494
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10411 | RAPGEF3 | Rap guanine nucleotide exch... | XM_017018688.2 | 62.4% | 60.7% | (many diffs) |
| 2 | human | 10411 | RAPGEF3 | Rap guanine nucleotide exch... | XR_001748551.2 | 37.7% | (many diffs) | |
| 3 | human | 10411 | RAPGEF3 | Rap guanine nucleotide exch... | NM_001098532.2 | 36.1% | 35.3% | (many diffs) |
| 4 | human | 10411 | RAPGEF3 | Rap guanine nucleotide exch... | NM_006105.5 | 36.1% | 35.3% | (many diffs) |
| 5 | human | 10411 | RAPGEF3 | Rap guanine nucleotide exch... | XM_011537755.1 | 35.5% | 34.5% | (many diffs) |
| 6 | human | 10411 | RAPGEF3 | Rap guanine nucleotide exch... | NM_001098531.3 | 34.5% | 33.6% | (many diffs) |
| 7 | human | 10411 | RAPGEF3 | Rap guanine nucleotide exch... | XM_011537752.2 | 33.9% | 33% | (many diffs) |
| 8 | human | 10411 | RAPGEF3 | Rap guanine nucleotide exch... | XR_002957282.1 | 27.6% | (many diffs) | |
| 9 | human | 10411 | RAPGEF3 | Rap guanine nucleotide exch... | XR_001748550.2 | 27.1% | (many diffs) | |
| 10 | human | 10411 | RAPGEF3 | Rap guanine nucleotide exch... | XM_011537758.2 | 24.5% | 20.7% | (many diffs) |
| 11 | mouse | 223864 | Rapgef3 | Rap guanine nucleotide exch... | XR_875293.1 | 34.9% | (many diffs) | |
| 12 | mouse | 223864 | Rapgef3 | Rap guanine nucleotide exch... | XR_875292.1 | 34.8% | (many diffs) | |
| 13 | mouse | 223864 | Rapgef3 | Rap guanine nucleotide exch... | XM_011245602.2 | 31.3% | 32.1% | (many diffs) |
| 14 | mouse | 223864 | Rapgef3 | Rap guanine nucleotide exch... | NM_001177811.1 | 30.5% | 31.3% | (many diffs) |
| 15 | mouse | 223864 | Rapgef3 | Rap guanine nucleotide exch... | NM_144850.2 | 30.2% | 31% | (many diffs) |
| 16 | mouse | 223864 | Rapgef3 | Rap guanine nucleotide exch... | NM_001177810.1 | 30% | 30.7% | (many diffs) |
| 17 | mouse | 223864 | Rapgef3 | Rap guanine nucleotide exch... | XM_011245600.1 | 29.9% | 30.6% | (many diffs) |
| 18 | mouse | 223864 | Rapgef3 | Rap guanine nucleotide exch... | XM_011245601.2 | 29.9% | 30.6% | (many diffs) |
| 19 | mouse | 223864 | Rapgef3 | Rap guanine nucleotide exch... | XM_006520876.2 | 29.3% | 30.1% | (many diffs) |
| 20 | mouse | 223864 | Rapgef3 | Rap guanine nucleotide exch... | XR_875291.2 | 29.3% | (many diffs) | |
| 21 | mouse | 223864 | Rapgef3 | Rap guanine nucleotide exch... | XM_017316584.1 | 29.2% | 30% | (many diffs) |
| 22 | mouse | 223864 | Rapgef3 | Rap guanine nucleotide exch... | XM_017316583.1 | 29% | 29.7% | (many diffs) |
| 23 | mouse | 223864 | Rapgef3 | Rap guanine nucleotide exch... | XM_017316582.1 | 28.9% | 29.6% | (many diffs) |
| 24 | mouse | 223864 | Rapgef3 | Rap guanine nucleotide exch... | XR_001781519.1 | 22.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1029
- ORF length:
- 963
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt gttgagaagg atgcaccggc cccgaagctg ctcctaccag ctgctgctgg 121 agcaccagcg tccgagctgc atccaggggc tgcgctggac accactcacc aacagcgagg 181 agtccctgga tttcagcgag agcctggagc aggcctccac agagcgggtg ctcagggctg 241 ggaggcagct gcatcggcat ctgctggcca cctgcccaaa cctcatccga gaccggaagt 301 accaccttag gctctatcgg cagtgctgct ctggccggga gctggtggat gggatcttgg 361 ccctgggact tggggtccat tcccggagcc aagttgtggg aatctgccag gtgctgctgg 421 atgaaggtgc cctctgccat gtgaaacacg actgggcctt ccaggaccga gatgcccaat 481 tctaccggtt ccccgggccc gagcccgagc ccgtgggaac tcatgagatg gaggaggagt 541 tggccgaagc tgtggccctg ctctcccagc gggggcctga cgccatgctc actgtggcac 601 ttcgaaagcc cccaggtcag cgcacggatg aagagctgga cctcatcttt gaggagctgc 661 tgcacatcaa ggctgtggcc cacctctcca actcggtgaa gcgagaatta gcggctgttc 721 tgctctttga accacacagc aaggcaggga ccgtgttgtt cagccagggg gacaagggca 781 cttcgtggta cattatctgg aagggatctg tcaacgtggt gacccatggc aaggggctgg 841 tgaccaccct gcatgaggga gatgattttg gacagctggc tctggtgaat gatgcacccc 901 gggcagccac catcatcctg cgagaagaca actgtcattt cctgcgtgtg gacaagcagg 961 acttcaaccg tatcatcaag gtgctgctgc tggggttggg gagggcaggg gccattcgtc 1021 cagagaagtg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1081 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1141 tcaacctctg gatta