Construct: ORF ccsbBroad304_11532
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009574.1_s304c1, BRDN0000409091
- Derived from:
- ccsbBroadEn_11532
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDPN (10630)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11532
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10630 | PDPN | podoplanin | NM_001006624.2 | 73.8% | 73.4% | 0_1ins126;314C>G |
| 2 | human | 10630 | PDPN | podoplanin | XM_006710295.1 | 73.8% | 73.4% | 0_1ins126;314C>G |
| 3 | human | 10630 | PDPN | podoplanin | XM_024451404.1 | 73.8% | 73.4% | 0_1ins126;314C>G |
| 4 | human | 10630 | PDPN | podoplanin | NM_001006625.2 | 72.6% | 71.6% | 0_1ins126;314C>G;350_351insGTACTC |
| 5 | human | 10630 | PDPN | podoplanin | XM_024451415.1 | 72.6% | 71.6% | 0_1ins126;314C>G;350_351insGTACTC |
| 6 | human | 10630 | PDPN | podoplanin | NM_006474.4 | 67.9% | 67.6% | 1_228del;668C>G |
| 7 | human | 10630 | PDPN | podoplanin | NM_198389.2 | 67% | 66.3% | 1_228del;668C>G;704_705insGTACTC |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 552
- ORF length:
- 486
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtg gaaggtgtca gctctgctct tcgttttggg aagcgcgtcg ctctgggtcc 121 tggcagaagg agccagcaca ggccagccag aagatgacac tgagactaca ggtttggaag 181 gcggcgttgc catgccaggt gccgaagatg atgtggtgac tccaggaacc agcgaagacc 241 gctataagtc tggcttgaca actctggtgg caacaagtgt caacagtgta acaggcattc 301 gcatcgagga tctgccaact tcagaaagca cagtccacgc gcaagaacaa agtccaagcg 361 ccacagcctc aaacgtggcc accagtcact ccacggagaa agtggatgga gacacacaga 421 caacagttga gaaagatggt ttgtcaacag tgaccctggt tggaatcata gttggggtct 481 tactagccat cggcttcatt ggtggaatca tcgttgtggt tatgcgaaaa atgtcgggaa 541 ggtactcgcc ctacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 601 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 661 caatcaacct ctggatta