Construct: ORF ccsbBroad304_11536
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005413.1_s304c1, BRDN0000399491
- Derived from:
- ccsbBroadEn_11536
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CGREF1 (10669)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11536
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10669 | CGREF1 | cell growth regulator with ... | NM_001166239.2 | 94.4% | 94.6% | 348C>A;375C>G;709_759del |
| 2 | human | 10669 | CGREF1 | cell growth regulator with ... | NM_006569.6 | 94.4% | 94.6% | 348C>A;375C>G;709_759del |
| 3 | human | 10669 | CGREF1 | cell growth regulator with ... | NM_001166240.1 | 42.8% | 39.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 969
- ORF length:
- 903
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt acctttgacg atgacagtgt taatcctgct gctgctcccc acgggtcagg 121 ctgccccaaa ggatggagtc acaaggccag actctgaagt gcagcatcag ctcctgccca 181 accccttcca gccaggccag gagcagctcg gacttctgca gagctaccta aagggactag 241 gaaggacaga agtgcaactg gagcatctga gccgggagca ggttctcctc tacctctttg 301 ccctccatga ctatgaccag agtggacagc tggatggcct ggagctgctg tccatgttga 361 cagctgctct ggcccctgga gctgccaact ctcctaccac caacccggtg atattgatag 421 tggacaaagt gctcgagacg caggacctga atggggatgg gctcatgacc cctgctgagc 481 tcatcaactt cccgggagta gccctcaggc acgtggagcc cggagagccc cttgctccat 541 ctcctcagga gccacaagct gttggaaggc agtccctatt agctaaaagc ccattaagac 601 aagaaacaca ggaagcccct ggtcccagag aagaagcaaa gggccaggta gaggccagaa 661 gggagtcttt ggatcctgtc caggagcctg ggggccaggc agaggctgat ggagatgttc 721 cagggcccag aggggaagct gagggccagg cagaggctaa aggagatgcc cctgggccca 781 gaggggaagc tgggggccag gcagaggctg aaggagatgc ccccgggccc agaggggaag 841 ctgggggcca ggcagaggcc agggagaatg gagaggaggc caaggaactt ccaggggaaa 901 cactggagtc taagaacacc caaaatgact ttgaggtgca cattgttcaa gtggagaatg 961 atgagatctg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1021 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1081 tcaacctctg gatta