Construct: ORF ccsbBroad304_11589

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011306.1_s304c1, BRDN0000401885
Derived from:
ccsbBroadEn_11589
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TOPBP1 (11073)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11589
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11073 TOPBP1 DNA topoisomerase II bindin... NM_007027.4 94.2% 94.2% 1_261del
2 human 11073 TOPBP1 DNA topoisomerase II bindin... XM_017005636.2 94.2% 94.2% 1_261del
3 human 11073 TOPBP1 DNA topoisomerase II bindin... NM_001363889.2 93.9% 93.8% 1_261del;922_923insGTCGTACTCTTTCAG
4 human 11073 TOPBP1 DNA topoisomerase II bindin... XR_001739988.2 80.3% 1_359del;3619_3620ins70;4595_5203del
5 human 11073 TOPBP1 DNA topoisomerase II bindin... XM_017005637.2 75.7% 75.7% 0_1ins1044
6 human 11073 TOPBP1 DNA topoisomerase II bindin... XM_011512357.3 68.2% 68% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
4374
ORF length:
4305
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gcaccaccag cgatgtgtcc caagagccga acatccagtt tataatatgg
121ttatgtctga tgtaaccata tcttgtacaa gtctggaaaa agaaaaaagg gaagaagttc
181ataaatatgt acaaatgatg ggcggacgag tatacagaga ccttaatgta tcagtaactc
241accttattgc aggagaagtt ggtagcaaaa aatatttagt tgctgcaaac ctgaagaaac
301ctattttgct tccctcttgg ataaaaacac tttgggagaa gtcacaagag aaaaaaataa
361ctagatatac tgatataaac atggaagatt tcaagtgtcc tatttttctt ggttgcataa
421tctgtgtgac tggcttatgt ggcttagaca ggaaagaagt tcagcaactc acagttaagc
481atggaggtca atacatggga caattgaaaa tgaatgaatg tacacacctc attgtgcaag
541aaccaaaagg tcagaagtat gagtgtgcca agagatggaa tgtacactgt gtgaccacac
601agtggttttt tgacagtatt gagaaaggtt tttgtcagga tgaatccata tacaagacag
661aacctagacc agaagcaaag actatgccca attcttcaac tcctaccagc cagatcaaca
721caattgatag tcgtactctt tcagatgtca gcaatatttc caacataaat gcaagttgcg
781taagtgaatc aatatgtaat tcacttaaca gcaaactgga gcctacactt gaaaatctag
841aaaatctgga tgtcagtgca tttcaagcac ctgaagattt attagatggt tgtcggatat
901atctttgcgg ttttagtggc agaaagctag ataaactgag aagacttatt aacagtggag
961gtggagttcg ttttaaccag ctaaatgaag atgtaactca tgttattgtg ggagattatg
1021atgatgaatt gaagcagttt tggaataaat cagcccacag gcctcatgta gtgggagcaa
1081agtggttgct agagtgtttc agtaaaggtt atatgctttc tgaagaacca tatatccatg
1141ctaattacca gccagtggaa attccagttt cacataagcc tgaaagtaaa gcagctcttt
1201taaaaaagaa gaacagcagc ttctctaaga aagactttgc tcctagtgaa aagcatgagc
1261aagctgatga agatctgctc tctcaatatg aaaatggtag ctccacagta gttgaggcta
1321agacgtctga agccaggccc tttaatgatt ctactcatgc tgagcccttg aatgattcta
1381ctcacatttc tttgcaagaa gaaaaccagt cttctgtcag tcattgtgtc cctgatgttt
1441ctacaattac tgaagaaggc ttatttagcc aaaagagttt ccttgttttg ggttttagta
1501atgaaaatga atctaacatc gcaaacatca taaaagaaaa tgctgggaaa atcatgtccc
1561ttctgagcag aactgttgcg gattatgctg tggttcctct gctggggtgt gaagtggaag
1621ccactgtggg agaagttgtt acaaatacat ggctggttac ttgcatagac tatcagactt
1681tgtttgatcc aaagtcgaat cctctcttca caccagttcc agtaatgaca ggaatgactc
1741ctttagagga ttgtgttatt tcatttagcc agtgtgctgg agcagaaaaa gagtctttaa
1801cattcctagc aaacctcctt ggagcaagtg ttcaagaata ctttgttcgc aaatccaatg
1861caaagaaagg catgtttgcc agtactcatc ttatactgaa agaacgtggt ggctctaaat
1921atgaagctgc aaagaagtgg aatttacctg ccgttactat agcttggctg ttggagactg
1981ctagaacggg aaagagagca gacgaaagcc attttctgat tgaaaattca actaaagaag
2041aacgaagttt ggaaacagaa ataacaaatg gaatcaatct aaattcagat actgcagagc
2101atcctggcac acgcctgcaa actcacagaa aaaccgtcgt tacaccttta gatatgaacc
2161gctttcagag taaagctttc cgtgctgtgg tctcacaaca tgccagacag gtcgcagcct
2221ccccagcagt aggacaacca cttcagaagg agccctcgtt acacctggat acaccatcaa
2281aattcctgtc caaggacaaa ctcttcaagc cttcctttga tgtgaaggat gcacttgcag
2341ccttggaaac tccaggacgt cccagccaac agaaaaggaa accgagtacg ccactctcag
2401aagttattgt caaaaacttg caacttgctt tggcaaatag ctctcgaaat gctgtcgctc
2461tttctgccag ccctcaactg aaagaggccc agtcagagaa ggaagaagcc ccaaagccac
2521ttcacaaagt agtggtatgt gttagtaaaa aactcagtaa gaagcagagt gaactaaatg
2581ggatcgcagc ctctctagga gcagattaca ggtggagttt tgatgaaaca gtgactcatt
2641tcatctatca agggcggcca aatgacacta atcgggagta taaatctgta aaagaaagag
2701gagtacacat tgtttccgag cactggcttt tagattgtgc ccaagagtgt aaacatcttc
2761ctgaatctct ttatccacat acttataatc ccaaaatgag cttggatatc agcgcagtgc
2821aagatggccg gctctgtaat agtcgactac tctcagctgt gtcttcaaca aaggatgatg
2881agccagatcc tttgatttta gaagaaaatg atgtagacaa tatggccacc aataataaag
2941agtcagcacc atcaaatgga agtggaaaga atgactctaa aggagttctg acacagacct
3001tagagatgag agagaacttt cagaagcagt tacaggagat aatgtctgca acatcaatag
3061tgaaacccca agggcagagg acttcccttt caagaagtgg ttgtaacagc gcatcttcaa
3121cccctgacag cactcgctct gctcgcagtg gacgaagtag agtcctagag gcactgaggc
3181agtctcgtca gacagtacct gatgtcaaca cagagccttc ccaaaatgaa cagatcattt
3241gggatgaccc tacagcaagg gaggagagag caaggcttgc cagcaatttg cagtggccta
3301gttgtcccac acaatactct gagcttcagg ttgacattca aaacttggag gattctcctt
3361ttcaaaagcc tttacatgat tcagaaattg ctaaacaggc tgtctgtgat cctggaaaca
3421tacgtgtgac tgaagctccc aaacacccaa tctctgaaga actggaaact cccataaaag
3481acagccacct gatccctacg cctcaagccc ccagtattgc ctttccactc gccaaccccc
3541ctgtggctcc gcaccctaga gaaaagatta taacgataga ggagactcat gaagaattaa
3601aaaaacagta catatttcag ttatcatctc tgaatcctca agaacgtatt gactattgtc
3661atctgattga gaaactaggt ggattggtga tagaaaagca gtgctttgat cccacctgta
3721cacacattgt tgtgggacat ccacttcgaa acgagaagta tttagcctca gtggcagctg
3781ggaagtgggt gcttcatcgc tcctaccttg aagcctgcag gactgctgga cacttcgtgc
3841aggaagaaga ctatgaatgg ggaagtagtt ccatacttga tgttctgact ggaatcaatg
3901tacagcaacg aagactagca cttgcagcaa tgagatggag aaaaaaaatc cagcaaagac
3961aagaatctgg cattgttgag ggagcattta gtgggtggaa ggttatttta catgtggatc
4021agtctcgaga agcaggcttc aaacgccttc ttcagtcagg aggagcaaag gtgctacctg
4081gtcattctgt acctttattt aaagaggcca cacatctttt ttctgacttg aataaactga
4141aaccagatga ctcaggagtt aatatagcag aagctgctgc ccagaacgtg tactgcttga
4201gaacagaata cattgctgat tatctcatgc aggaatcacc tcctcatgta gaaaattact
4261gtctaccaga agctatttca tttattcaga ataataagga acttgggact ggattatcac
4321aaaagaggaa agctcctaca gaaaaaaata aaatcaaacg acctagagta cacttgccaa
4381ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
4441cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)

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