Construct: ORF ccsbBroad304_11597
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012213.1_s304c1, BRDN0000408630
- Derived from:
- ccsbBroadEn_11597
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRDM5 (11107)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11597
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11107 | PRDM5 | PR/SET domain 5 | NM_001300823.1 | 99.9% | 100% | 1141T>C |
| 2 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_011531563.2 | 98.1% | 98.1% | 936_968del;1174T>C |
| 3 | human | 11107 | PRDM5 | PR/SET domain 5 | NM_018699.3 | 95% | 95% | 651_743del;1234T>C |
| 4 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_011531562.2 | 93.3% | 93.4% | 651_743del;1029_1061del;1267T>C |
| 5 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_017007668.2 | 89.1% | 88.6% | (many diffs) |
| 6 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_011531564.2 | 87.9% | 87.9% | (many diffs) |
| 7 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_011531566.3 | 85.2% | 85% | (many diffs) |
| 8 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_011531565.2 | 85.1% | 85% | (many diffs) |
| 9 | human | 11107 | PRDM5 | PR/SET domain 5 | NM_001300824.1 | 83.5% | 78.9% | 1141T>C;1443_1444ins86;1503_1504ins208 |
| 10 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_017007669.1 | 79.4% | 75.1% | (many diffs) |
| 11 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_017007670.1 | 79.4% | 75.1% | (many diffs) |
| 12 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_011531567.2 | 78.1% | 73.9% | (many diffs) |
| 13 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_011531568.2 | 78.1% | 73.9% | (many diffs) |
| 14 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_011531569.3 | 78.1% | 73.9% | (many diffs) |
| 15 | human | 11107 | PRDM5 | PR/SET domain 5 | XR_938677.3 | 75.8% | (many diffs) | |
| 16 | human | 11107 | PRDM5 | PR/SET domain 5 | XR_938680.2 | 67.8% | (many diffs) | |
| 17 | human | 11107 | PRDM5 | PR/SET domain 5 | XR_938678.1 | 66.7% | (many diffs) | |
| 18 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_024453879.1 | 65.3% | 58% | 0_1ins499;151_152ins122;520T>C |
| 19 | human | 11107 | PRDM5 | PR/SET domain 5 | XR_938679.1 | 64.6% | (many diffs) | |
| 20 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_011531570.3 | 64.2% | 57% | (many diffs) |
| 21 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_017007671.1 | 49.9% | 50% | 0_1ins882;54_86del;292T>C |
| 22 | human | 11107 | PRDM5 | PR/SET domain 5 | XM_005262708.3 | 49.8% | 49.5% | (many diffs) |
| 23 | mouse | 70779 | Prdm5 | PR domain containing 5 | NM_027547.2 | 87.7% | 92.6% | (many diffs) |
| 24 | mouse | 70779 | Prdm5 | PR domain containing 5 | XM_006506604.3 | 83.3% | 88% | (many diffs) |
| 25 | mouse | 70779 | Prdm5 | PR domain containing 5 | XM_011241464.2 | 74.1% | 78.9% | (many diffs) |
| 26 | mouse | 70779 | Prdm5 | PR domain containing 5 | NM_029549.1 | 62.2% | 67.7% | (many diffs) |
| 27 | mouse | 70779 | Prdm5 | PR domain containing 5 | XR_377493.3 | 58.1% | (many diffs) | |
| 28 | mouse | 70779 | Prdm5 | PR domain containing 5 | XM_006506602.3 | 50.5% | 54.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1866
- ORF length:
- 1797
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgggcatg tacgtgccgg acaggttctc cctgaagtcc tcccgggttc 121 aggacggcat ggggctctac acggcccgca gagtgcgaaa gggtgaaaag ttcggaccct 181 ttgctggaga gaagagaatg cctgaagact tggatgaaaa tatggattac aggttgatgt 241 gggaggttcg tgggagtaag ggagaagttt tgtacatttt ggatgctacc aacccacggc 301 actccaactg gcttcgcttc gttcatgagg caccatctca ggagcagaag aacttggctg 361 ccattcaaga aggagaaaac attttctatt tggcagttga agatatagaa acagacacgg 421 agcttctgat tggctacctg gatagtgaca tggaggctga ggaggaagaa cagcaaatta 481 tgacagtcat caaagaaggg gaagttgaaa attctagaag acaatcaaca gcgggcagaa 541 aagatcgcct tggctgtaaa gaggactatg cttgtcctca atgtgaatcg agttttacca 601 gtgaggatat tcttgctgag catctccaga cattgcacca gaaacccaca gaggagaaag 661 aatttaagtg caagaactgt gggaagaaat tcccagttaa gcaggctttg caaagacatt 721 ttgagcagca ccaggagact tgccgggggg atgccaggtt tgtgtgcaag gctgacagct 781 gtggaaagag gctgaagagc aaggatgccc tgaaaagaca ccaggaaaat gtccacactg 841 gagatcctaa gaaaaagctt atatgttcag tgtgcaataa aaagtgttct tcagcatcaa 901 gcctacagga acatagaaag attcatgaga tatttgattg tcaagaatgt atgaagaaat 961 ttatttcagc taatcagcta aaacgtcata tgatcaccca ctcagaaaaa cgaccctata 1021 attgcgagat ttgtaataag tctttcaaga ggcttgatca agtgggtgct cacaaagtaa 1081 tacacagcga agacaaacct tacaaatgca aactttgtgg aaagggattt gcccacagaa 1141 atgtttacaa gaatcataag aagacccact ctgaggagag accgttccaa tgtgaagaat 1201 gtaaagctct gttccggacc ccattttctt tacagagaca cctgctaata cataacagtg 1261 agaggacttt caagtgccat cactgcgatg ctacctttaa gaggaaggat acattaaatg 1321 ttcatgtcca ggtggttcat gaaagacaca agaagtatag gtgtgagcta tgtaataagg 1381 cctttgttac accttcagtg cttagaagtc ataagaaaac acatacagga gaaaaggaga 1441 aaatctgtcc atattgtggc cagaaatttg ccagcagtgg tacactcaga gttcatatcc 1501 ggagccacac aggtgagcgt ccctatcaat gtccttactg tgaaaaagga ttcagtaaaa 1561 atgatggact gaagatgcac attcgtactc acaccaggga gaagccgtac aagtgctcag 1621 agtgcagcaa ggccttcagc cagaagcgag gcctggatga gcacaagagg acgcacactg 1681 gagaaaagcc ttttcagtgt gatgtttgtg atttggcttt tagcctgaag aaaatgctga 1741 ttcgacacaa gatgactcat aatcccaatc gtcccctggc agaatgccag ttttgccata 1801 agaagtttac aaggaatgac tacctcaaag tgcacatgga caatatccat ggtgtagctg 1861 acagcttgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 1921 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1981 acctctggat ta