Construct: ORF ccsbBroad304_11636

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009953.1_s304c1, BRDN0000398747
Derived from:
ccsbBroadEn_11636
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CILK1 (22858)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11636
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 22858 CILK1 ciliogenesis associated kin... NM_014920.4 45.1% 44.7% (many diffs)
2 human 22858 CILK1 ciliogenesis associated kin... NM_016513.4 45.1% 44.7% (many diffs)
3 human 22858 CILK1 ciliogenesis associated kin... XM_017010488.1 45.1% 44.7% (many diffs)
4 human 22858 CILK1 ciliogenesis associated kin... XM_017010489.1 45.1% 44.7% (many diffs)
5 human 22858 CILK1 ciliogenesis associated kin... XM_017010490.1 45.1% 44.7% (many diffs)
6 human 22858 CILK1 ciliogenesis associated kin... XM_017010491.1 45.1% 44.7% (many diffs)
7 human 22858 CILK1 ciliogenesis associated kin... XM_017010492.1 45.1% 44.7% (many diffs)
8 human 22858 CILK1 ciliogenesis associated kin... XM_011514419.1 44.6% 44.2% (many diffs)
9 human 22858 CILK1 ciliogenesis associated kin... XM_011514420.2 44.6% 44.2% (many diffs)
10 human 22858 CILK1 ciliogenesis associated kin... XM_011514421.1 44.6% 44.2% (many diffs)
11 human 22858 CILK1 ciliogenesis associated kin... XM_017010485.1 44.6% 44.2% (many diffs)
12 human 22858 CILK1 ciliogenesis associated kin... XM_017010486.1 44.6% 44.2% (many diffs)
13 human 22858 CILK1 ciliogenesis associated kin... XM_017010487.1 44.6% 44.2% (many diffs)
14 mouse 56542 Ick intestinal cell kinase NM_001163780.1 40.5% 43.2% (many diffs)
15 mouse 56542 Ick intestinal cell kinase NM_019987.2 40.5% 43.2% (many diffs)
16 mouse 56542 Ick intestinal cell kinase XM_006511304.2 40.5% 43.2% (many diffs)
17 mouse 56542 Ick intestinal cell kinase XM_006511305.3 29% 30.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
942
ORF length:
876
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa tagatacaca acaatcaggc agctcgggga tggaacctac ggttccgtcc
121tgctgggaag aagcattgag tctggggagc tgatcgctat taaaaaaatg aaaagaaaat
181tttattcctg ggaggaatgc atgaaccttc gggaggttaa gtctttaaag aagctcaacc
241atgccaatgt agtcaaatta aaagaagtta tcagggaaaa tgatcatctt tattttatct
301tcgagtacat gaaggaaaat ctttaccagc tcattaaaga gagaaataag ttgtttcctg
361agtctgctat aaggaatatc atgtatcaga tattacaagg actcgcattt attcacaaac
421acggcttctt tcatcgagac ttaaagcctg agaacctcct ctgcatggga ccagaacttg
481tgaaaattgc agactttggt ttggcccgag aaatacgatc aaaacctcca tatacagatt
541atgtatctac cagatggtac agggctccag aagtactcct gaggtctacc aactacagct
601cccccattga cgtctgggcg gtgggctgca tcatggcaga agtttacacc ctcaggccac
661tcttccctgg agccagtgaa attgacacaa tattcaaaat ttgccaagtg ctggggacac
721caaaaaagac tgactggcct gaaggctatc aactttcaag tgcaatgaac ttccgttggc
781cacagtgtgt acccaataac ttaaagacct tgattcccaa tgctagcagt gaagcagtcc
841agctcctgag agacatgctt cagtgggatc ccaagaaacg accaacagct agtcaggttt
901tcttccattt tcttgttata acgttcatat ctaactctga atacccaact ttcttgtaca
961aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1021ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)