Construct: ORF ccsbBroad304_11727
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013607.1_s304c1, BRDN0000407245
- Derived from:
- ccsbBroadEn_11727
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FNBP4 (23360)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11727
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23360 | FNBP4 | formin binding protein 4 | NM_015308.5 | 99.8% | 99.8% | 166_171delACCACC |
| 2 | human | 23360 | FNBP4 | formin binding protein 4 | NM_001318339.3 | 99.6% | 99.6% | 166_171delACCACC;220_225delGGCAAG |
| 3 | human | 23360 | FNBP4 | formin binding protein 4 | XR_001747813.1 | 72% | (many diffs) | |
| 4 | human | 23360 | FNBP4 | formin binding protein 4 | XR_001747804.1 | 71.8% | (many diffs) | |
| 5 | human | 23360 | FNBP4 | formin binding protein 4 | XR_001747810.1 | 71.8% | (many diffs) | |
| 6 | human | 23360 | FNBP4 | formin binding protein 4 | XR_930855.2 | 68.6% | (many diffs) | |
| 7 | human | 23360 | FNBP4 | formin binding protein 4 | XR_002957135.1 | 68.4% | (many diffs) | |
| 8 | human | 23360 | FNBP4 | formin binding protein 4 | XM_024448408.1 | 66.3% | 65.5% | (many diffs) |
| 9 | human | 23360 | FNBP4 | formin binding protein 4 | XM_024448409.1 | 66.3% | 65.6% | (many diffs) |
| 10 | human | 23360 | FNBP4 | formin binding protein 4 | XR_930854.1 | 65.3% | (many diffs) | |
| 11 | human | 23360 | FNBP4 | formin binding protein 4 | XR_002957134.1 | 60.1% | (many diffs) | |
| 12 | human | 23360 | FNBP4 | formin binding protein 4 | XR_242785.2 | 57.1% | (many diffs) | |
| 13 | human | 23360 | FNBP4 | formin binding protein 4 | XR_002957136.1 | 54.7% | (many diffs) | |
| 14 | human | 23360 | FNBP4 | formin binding protein 4 | XR_001747815.1 | 53.7% | (many diffs) | |
| 15 | mouse | 55935 | Fnbp4 | formin binding protein 4 | NM_018828.2 | 80.7% | 81.7% | (many diffs) |
| 16 | mouse | 55935 | Fnbp4 | formin binding protein 4 | XR_374493.2 | 59.2% | (many diffs) | |
| 17 | mouse | 55935 | Fnbp4 | formin binding protein 4 | XM_006499919.2 | 54.8% | 54.5% | (many diffs) |
| 18 | mouse | 55935 | Fnbp4 | formin binding protein 4 | XR_866324.1 | 54.2% | (many diffs) | |
| 19 | mouse | 55935 | Fnbp4 | formin binding protein 4 | XR_001783149.1 | 50.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3111
- ORF length:
- 3045
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg gaagaagtcc cgggcggtac ccggccgtag gcccatcctg caactctctc 121 cgccgggtcc tcggggcagc acgccgggcc gggacccgga gccggaaccc gacactgagc 181 cggactcaac cgcggcggtc cccagccagc ccgccccgtc ggcggcgacg accaccgcgg 241 tgactgccgc cgcggcctcg gacgactcgc cttcagaaga tgaacaggaa gcggtgcagg 301 aggttcctag agttgttcag aatcctccaa aaccagtcat gaccactaga cccacagctg 361 ttaaagcaac aggcggtcta tgcttgcttg gtgcttatgc tgacagtgat gacgatgaca 421 atgatgtttc cgaaaaacta gcacaatcca aagagacaaa tggaaaccag tcaactgata 481 ttgatagtac attggccaac ttcctagcgg agatcgatgc cataacagct cctcagcctg 541 cagctcctgt aggagcttct gctccacctc caactccacc tcgaccagag ccaaaggaag 601 cagcaacatc taccctttct tcttctactt caaatggaac agactccacc caaacatctg 661 gttggcaata tgatactcag tgttcactgg caggagtcgg aattgagatg ggcgattggc 721 aggaagtctg ggatgagaac acgggatgtt attattattg gaatacacaa acaaatgaag 781 tgacttggga gttaccccaa tatcttgcca cacaggtaca gggattacag cattaccagc 841 ccagttctgt gccaggtgct gaaactagtt ttgtggtaaa tacagacata tattctaagg 901 agaaaacgat ttctgtttcc agtagtaaaa gtggaccagt catagccaag cgagaagtta 961 aaaaggaagt aaatgaagga attcaggctc tctcaaatag tgaggaggag aagaaagggg 1021 tggcagcatc gctgcttgct cctttattgc ctgagggaat aaaagaagaa gaagagagat 1081 ggagaagaaa agtaatttgt aaagaggagc cagtttcaga agtaaaagaa acaagtacaa 1141 cagtagaaga agcaacaaca atagtaaagc cacaggaaat tatgttggac aatatagaag 1201 acccttctca ggaggatctt tgcagtgttg tccaatctgg agaaagtgag gaggaagagg 1261 aacaagatac ccttgaactg gagctagttt tggaaaggaa aaaagcagag ttgcgagcct 1321 tggaggaagg agatggtagt gtgtcagggt ctagtccacg ttctgatatc agccagccag 1381 catctcaaga tggaatgcgt aggcttatgt ctaaaagagg aaaatggaag atgtttgttc 1441 gagctaccag tccagaatct accagtagga gttctagtaa aactggacga gatactccag 1501 aaaatggaga aactgcaatt ggtgctgaaa attcagaaaa aatagatgag aattcagata 1561 aagagatgga agtagaagaa tctccagaga aaataaaagt acagacaaca ccaaaagtag 1621 aagaagaaca ggatttgaaa tttcagattg gagaactggc aaataccctg acaagtaaat 1681 tcgagtttct aggcattaat agacaatcca tctccaactt tcatgtgctg ctcttacaga 1741 ctgagactcg aattgcagac tggcgggaag gggctcttaa tggaaactac cttaaacgaa 1801 aacttcagga tgcagcagaa caactaaaac agtatgaaat aaacgccact cctaaaggct 1861 ggtcctgcca ctgggacagg gatcatagac ggtatttcta tgtaaacgaa cagtcgggcg 1921 agtctcagtg ggagtttcca gatggtgaag aggaagaaga agaaagccaa gcacaagaaa 1981 atagagatga gactcttgcc aaacagacct tgaaagacaa aactggcact gattcaaatt 2041 caacagaatc ctctgaaact tccacaggtt ctctttgtaa agaatccttt tctggtcaag 2101 tttcttcttc atcactcatg ccacttactc cattctggac cctccttcag tcaaatgttc 2161 ctgtgcttca acctccatta cccttggaaa tgccaccacc cccacctcca cctccagaat 2221 cacctccacc ccctcctcca ccacctcctc ctgcggaaga tggtgagatc caggaggtag 2281 agatggagga tgagggaagt gaggagcccc ctgccccagg aacagaggaa gatacccctt 2341 tgaaaccttc agcacaaacc acagttgtaa ctagccagag ttcagttgat tccaccatct 2401 ctagttcttc ttccactaaa ggaataaaga ggaaagctac agaaattagc actgcagtgg 2461 ttcagaggtc agctaccatt ggcagttctc cagttctcta tagccagtca gctatagcta 2521 caggtcacca ggcagcaggg attggaaacc aggcaacagg aattggacat cagacaatac 2581 cagttagcct tccagcagca ggaatgggtc atcaggccag aggaatgagc ctgcagtcaa 2641 attaccttgg actagcggca gcacctgcaa ttatgagtta tgcagaatgt tctgtcccaa 2701 ttggagtgac tgctccctca ttgcagccag ttcaggcccg aggtgctgtg cctaccgcta 2761 ccattataga accaccacca ccacctcctc ctcctcctcc tccaccacca ccagctccca 2821 aaatgccacc acctgaaaag acaaaaaaag gaaggaaaga caaggcaaag aagagtaaga 2881 ccaaaatgcc atctttggta aaaaagtggc agagtatcca gcgtgagtta gatgaagagg 2941 acaattctag ttccagtgaa gaggatcggg aatcaactgc acagaagcga attgaagagt 3001 ggaaacagca gcagctggtt agtggcatgg cagagagaaa tgctaatttt gaagcccttc 3061 ctgaggattg gagagcaagg ctgaagagaa ggaaaatggc tccaaacaca tacccaactt 3121 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 3181 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta