Construct: ORF ccsbBroad304_11781

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009998.1_s304c1, BRDN0000405887
Derived from:
ccsbBroadEn_11781
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CCDC28A (25901)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11781
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 25901 CCDC28A coiled-coil domain containi... NM_015439.2 58.9% 58.3% 1_337del;342_343insA
2 human 25901 CCDC28A coiled-coil domain containi... XM_011535728.3 31.1% 30.6% 1_337del;342_343insA;594_594delTins229
3 mouse 215814 Ccdc28a coiled-coil domain containi... NM_144820.3 75.4% 82% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
552
ORF length:
486
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc aaaaaaaaat gccattccag tgagtaaaag cacagggttt tcaaatcctg
121catcacagtc aacttcacag cgaccaaagt taaaaagagt gatgaaagaa aagaccaaac
181ctcagggtgg agagggcaaa ggcgctcagt caactccgat ccagcactcc ttcctcactg
241atgtctcaga tgttcaggag atggagagag ggctgctcag tcttttgaat gatttccact
301ctggaaaact tcaagcattt ggaaatgaat gttccattga acagatggaa catgttcggg
361gaatgcagga gaaattagct cgcttgaatt tggagctcta tggggagtta gaggaacttc
421ctgaggataa gagaaaaaca gccagtgact ccaatctgga taggcttctg tcagatttag
481aagaattgaa ttcttccata caaaaactcc atttggcaga tgcacaagat gttccaaata
541cttctgctag ctacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
601tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
661caatcaacct ctggatta

Download FASTA (ORF) (Full)