Construct: ORF ccsbBroad304_11799
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004995.1_s304c1, BRDN0000462281
- Derived from:
- ccsbBroadEn_11799
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LRIG1 (26018)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11799
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26018 | LRIG1 | leucine rich repeats and im... | NM_015541.2 | 93.5% | 93.2% | (many diffs) |
| 2 | human | 26018 | LRIG1 | leucine rich repeats and im... | XM_017006134.2 | 91.3% | 91% | (many diffs) |
| 3 | human | 26018 | LRIG1 | leucine rich repeats and im... | XM_011533578.2 | 89.2% | 88.9% | (many diffs) |
| 4 | human | 26018 | LRIG1 | leucine rich repeats and im... | XM_017006135.1 | 72.3% | 69.6% | (many diffs) |
| 5 | human | 26018 | LRIG1 | leucine rich repeats and im... | XM_011533579.3 | 70.3% | 70.2% | 0_1ins780;380_381ins72;1151_1291del |
| 6 | human | 26018 | LRIG1 | leucine rich repeats and im... | XM_017006136.2 | 70.3% | 70.2% | 0_1ins780;380_381ins72;1151_1291del |
| 7 | mouse | 16206 | Lrig1 | leucine-rich repeats and im... | XM_006505621.2 | 74% | 73.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3276
- ORF length:
- 3210
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gcggccggtc cggggagggc tcggggcccc gcgccgctcg ccttgccttc 121 tccttctctg gctggttttg gttcggctgg agccggtgac cgccgcggcc ggcccgcggg 181 cgccctgcgc ggccgcctgc acttgcgctg gggactcgct ggactgcggt gggcgcgggc 241 tggctgcgtt gcccggggac ctgccctcct ggacgcggag cctaaacctg agttacaaca 301 aattctctga gattgaccct gctggttttg aggacttgcc gaacctacag gaagtgtacc 361 tcaataataa tgagttgaca gcggtaccat ccctgggcgc tgcttcatca catgtcgtct 421 ctctctttct gcagcacaac aagattcgca gcgtggaggg gagccagctg aaggcctacc 481 tttccttaga agtgttagat ctgagtttga acaacatcac ggaagtgcgg aacacctgct 541 ttccacacgg accgcctata aaggagctca acctggcagg caatcggatt ggcaccctgg 601 agttgggagc atttgatggt ctgtcacggt cgctgctaac tcttcgcctg agcaaaaaca 661 ggatcaccca gcttcctgta agagcattca agctacccag gctgacacaa ctggacctca 721 atcggaacag gattcggctg atagagggcc tcaccttcca ggggctcaac agcttggagg 781 tgctgaagct tcagcgaaac aacatcagca aactgacaga tggggccttc tggggactgt 841 ccaagatgca tgtgctgcac ctggagtaca acagcctggt agaagtgaac agcggctcgc 901 tctacggcct cacggccctg catcagctcc acctcagcaa caattccatc gctcgcattc 961 accgcaaggg ctggagcttc tgccagaagc tgcatgagtt ggtcctgtcc ttcaacaacc 1021 tgacacggct ggacgaggag agcctggccg agctgagcag cctgagtgtc ctgcgtctca 1081 gccacaattc catcagccac attgcggagg gtgccttcaa gggactcagg agcctgcgag 1141 tcttggatct ggaccataac gagatttcgg gcacaataga ggacacgagc ggcgccttct 1201 cagggctcga cagcctcagc aagctccttt tattagagcc atcccagtct gctggctgca 1261 gctccccgtc ccagccccat atgtctgctg ggggaaggac tctgtttgga aacaagatca 1321 agtctgtggc taagagagca ttctcggggc tggaaggcct ggagcacctg aaccttggag 1381 ggaatgcgat cagatctgtc cagtttgatg cctttgtgaa gatgaagaat cttaaagagc 1441 tccatatcag cagcgacagc ttcctgtgtg actgccagct gaagtggctg cccccgtggc 1501 taattggcag gatgctgcag gcctttgtga cagccacctg tgcccaccca gaatcactga 1561 agggtcagag cattttctct gtgccaccag agagtttcgt gtgcgatgac ttcctgaagc 1621 cacagatcat cacccagcca gaaaccacca tggctatggt gggcaaggac atccggttta 1681 catgctcagc agccagcagc agcagctccc ccatgacctt tgcctggaag aaagacaatg 1741 aagtcctgac caatgcagac atggagaact ttgtccacgt ccacgcgcag gacggggaag 1801 tgatggagta caccaccatc ctgcacctcc gtcaggtcac tttcgggcac gagggccgct 1861 accaatgtgt catcaccaac cactttggct ccacctattc acataaggcc aggctcaccg 1921 tgaatgtgtt gccatcattc accaaaacgc cccacgacat aaccatccgg accaccacca 1981 tggcccgcct cgaatgtgct gccacaggtc acccaaaccc tcagattgcc tggcagaagg 2041 atggaggcac ggatttcccc gctgcccaga ccccatcctt ggtggtcccc ttggaagacc 2101 gtgtggtatc tgtgggagaa acagtggccc tccaatgcaa agccacgggg aaccctccgc 2161 cccgcatcac ctggttcaag ggggaccgcc cgctgagcct cactgagcgg caccacttga 2221 cccctgacaa ccagctcctg gtggttcaga acgtggtggc agaggatgcg ggccgatata 2281 cctgtgagat gtccaacacc ctgggcacgg agcgagctca cagccagctg agcgtcctgc 2341 ccgcagcagg ctgcaggaag gatgggacca cggtaggcat cttcaccatt gctgtcgtga 2401 gcagcatcgt cctgacgtca ctggtctggg tgtgcatcat ctaccagacc aggaagaaga 2461 gtgaagagta cagtgtcacc aacacagatg aaaccgtcgt gccaccagat gttccaagct 2521 acctctcttc tcaggggacc ctttctgacc gacaagaaac cgtggtcagg accgagggtg 2581 gccctcaggc caatgggcac attgagagca atggtgtgtg tccaagagat gcaagccact 2641 ttccagagcc cgacactcac agcgttgcct gcaggcagcc aaagctctgt gctgggtctg 2701 cgtatcacaa agagccgtgg aaagcgatgg agaaagctga agggacacct gggccacata 2761 agatggaaca cggtggccgg gtcgtatgca gtgactgcaa caccgaagtg gactgttact 2821 ccaggggaca agccttccac ccccagcctg tgtccagaga cagcgcacag ccaagtgcgc 2881 caaatggccc ggagccgggt gggagtgacc aagagcattc tccacatcac cagtgcagca 2941 ggactgccgc tgggtcctgc cccgagtgcc aagggtcgct ctaccccagt aaccacgata 3001 gaatgctgac ggctgtgaag aaaaagccaa tggcatctct agatgggaaa ggggattctt 3061 cctggacttt agcaaggttg tatcacccgg actccacaga gctacagcct gcatcttcat 3121 taacttcagg cagtccagag cgcgcggaag cccagtactt gcttgtttcc aatggccacc 3181 tccccaaagc atgtgacgcc agtcccgagt ccacgccact gacaggacag ctccccggga 3241 aacagagggt gccactgctg ttggcaccaa aaagctacnc aactttcttg tacaaagtgg 3301 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 3361 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta