Construct: ORF ccsbBroad304_11801

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009812.1_s304c1, BRDN0000401899
Derived from:
ccsbBroadEn_11801
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PLEKHG3 (26030)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11801
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 26030 PLEKHG3 pleckstrin homology and Rho... NM_001308147.2 99.9% 100% 432T>C;729G>A;1791T>C
2 human 26030 PLEKHG3 pleckstrin homology and Rho... XM_017021157.2 99.9% 100% 432T>C;729G>A;1791T>C
3 human 26030 PLEKHG3 pleckstrin homology and Rho... XM_024449528.1 99.9% 100% 432T>C;729G>A;1791T>C
4 human 26030 PLEKHG3 pleckstrin homology and Rho... XM_011536629.3 97.5% 97.5% (many diffs)
5 human 26030 PLEKHG3 pleckstrin homology and Rho... XM_017021154.2 90.8% 90.9% (many diffs)
6 human 26030 PLEKHG3 pleckstrin homology and Rho... XM_011536625.3 90.8% 90.9% (many diffs)
7 human 26030 PLEKHG3 pleckstrin homology and Rho... XM_011536628.3 88.9% 88.9% (many diffs)
8 human 26030 PLEKHG3 pleckstrin homology and Rho... XM_017021155.2 88.9% 88.9% (many diffs)
9 human 26030 PLEKHG3 pleckstrin homology and Rho... XM_017021156.2 88.9% 88.9% (many diffs)
10 human 26030 PLEKHG3 pleckstrin homology and Rho... XM_011536627.3 87.5% 87.6% (many diffs)
11 human 26030 PLEKHG3 pleckstrin homology and Rho... XM_017021158.2 74.5% 74.5% (many diffs)
12 human 26030 PLEKHG3 pleckstrin homology and Rho... XR_002957542.1 36.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3726
ORF length:
3657
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gcctgtgtcc acctccctcc accaggatgg cagccaggag cggccggtga
121gcctgacctc taccacctcc tcgtcgggct cctcctgtga cagtcgcagt gccatggagg
181agcccagcag ctccgaggct cccgccaaga atggggcagg ctccctgaga agccggcatc
241tgcccaacag caacaacaac tccagcagct ggttgaacgt gaaggggccc ctctccccgt
301tcaacagccg ggcagcggca gggcctgcac accacaagct cagctacctg ggccgagtgg
361tgcgggagat cgtggagaca gagcgcatgt acgtacagga cctgcgcagc atcgtggagg
421actacctctt gaagatcatt gacacacccg ggctgctgaa gccagaacag gtcagcgccc
481tctttgggaa catagaaaac atctacgcgc tgaacagcca gctcctcaga gacctggaca
541gctgcaatag tgaccccgtg gctgtggcca gctgctttgt ggaaaggagc caagagtttg
601atatctacac tcagtattgc aacaattacc ccaactccgt ggccgccctg acggaatgca
661tgcgggacaa gcagcaggcc aagttctttc gggaccggca ggagctgcta cagcactcgc
721tgcccttggg ctcctacctg ctgaagccag tccagcgcat cctcaagtac cacctgctgc
781tccaggaaat tgccaaacat tttgatgaag aagaggatgg ctttgaggtg gtggaggatg
841ccattgacac catgacctgt gtggcctggt acatcaacga catgaagagg aggcatgagc
901acgcggtccg gctccaggag attcagtcac tcctcatcaa ctggaagggg cccgacctga
961ccacctacgg ggagcttgtc ctggagggca cattccgcgt gcatcgcgtg cgcaatgaaa
1021ggaccttttt cctctttgac aaaacactgc ttatcaccaa gaagcggggc gatcactttg
1081tctacaaggg caacatcccg tgctcctccc tgatgctgat cgaaagcacc agagactccc
1141tgtgcttcac tgtcacccac tacaagcaca gcaagcagca gtacagcatc caggccaaga
1201cagtggagga gaaacggaac tggactcacc acatcaagag gctcatccta gagaaccacc
1261atgccaccat tccccagaag gccaaggaag ccatcttgga aatggattcc tattatccca
1321atcggtaccg ctgcagccca gagcggctga agaaggcttg gtcctcccag gatgaggtgt
1381ccaccaatgt gcgccagggg cgccggcaat ctgagccaac caaacacctg ctcaggcaac
1441tcaacgagaa agcccgagca gcaggaatga agggaaaggg gcgcagggag tctgaaagct
1501ccaggagcag cagaaggccc agtggccggt ctccaaccag tactgagaag cgcatgagct
1561tcgagtccat ttcttccctg ccagaggttg agccggaccc tgaggctggg agtgagcaag
1621aggtattttc tgctgtggaa gggcccagtg ccgaggagac gccttcagac acagaatctc
1681cagaagtcct ggagacacag cttgatgccc accagggcct tctggggatg gaccccccag
1741gtgacatggt ggacttcgtg gcagctgaga gcactgagga ccttaaggcc ctgagcagcg
1801aggaggaaga agaaatggga ggtgccgccc aggagcctga gagccttctg ccaccctccg
1861tgctggacca ggccagcgtc attgcggagc gatttgtcag cagcttctct cggcggagca
1921gcgtggcaca ggaggacagc aagtccagtg gctttgggag cccgcggctg gtcagccgga
1981gcagcagcgt gctcagcctg gagggcagcg agaagggcct ggcccggcat ggcagtgcca
2041cagactccct cagctgtcag ctctccccag aagtggacat cagtgtgggg gtggccacag
2101aggacagccc ttctgtcaat gggatggagc ccccaagccc aggctgccca gtggagcctg
2161accggtcttc ctgcaagaag aaggaatcag cactctccac ccgagaccgg ctgttgctag
2221acaagattaa gagctattat gaaaatgcag aacaccatga tgcaggcttc agcgtccgtc
2281gccgggagag cctctcctac atccccaaag gactggtaag aaactccatc tccaggttca
2341acagccttcc ccggccagac ccagagccag tacctccagt ggggagcaag agacaggtgg
2401gctcccggcc gacttcgtgg gccctgtttg agctcccagg accaagccag gcagtcaaag
2461gggacccacc tcccatctca gatgctgagt tccgcccatc ttcagaaatt gtgaagatct
2521gggagggaat ggagtcttcc ggagggagcc ctgggaaggg gccaggccag ggccaggcca
2581atggctttga cctgcatgag ccactcttca tcctggagga gcatgagctg ggagccatca
2641cagaggagtc ggccactgcc tccccggaaa gctcctctcc cactgagggg cgcagcccgg
2701cccacctggc ccgggagctg aaagagctgg tgaaggagct gagcagcagt acccaggggg
2761agctggtggc cccactgcac ccccgcatcg tgcagctctc ccacgtaatg gacagccacg
2821tgagcgagcg cgtcaagaac aaggtctacc agctggcccg ccagtacagc ctccggatca
2881agagcaacaa gccagtgatg gccaggccac cactgcagtg ggaaaaggtg gcccctgaga
2941gggatgggaa gagccccact gtgccctgtc tacaggaaga ggctggagag ccattaggtg
3001gcaaaggtaa gaggaagccg gtgctgtctc tatttgacta tgagcagctg atggcccagg
3061agcacagccc tcccaagccc tcctcggctg gggagatgtc accacagcgt ttcttcttca
3121acccgtctgc tgtcagccag aggaccacct cgcctggggg ccggccctcc gcccggagcc
3181ccctcagccc cacagagacc ttcagctggc ccgacgtccg tgagctctgc tccaagtatg
3241cctcccgcga tgaggcacgc cgagcagggg gcggccggcc ccgcggccca cccgtcaaca
3301ggagccactc ggtgccggag aacatggtag agccacctct gtcgggcagg gtgggccgct
3361gccgcagcct gagcaccaag aggggccggg gaggcggaga ggctgcccaa tcccctgggc
3421ctctgcccca gagcaagccg gatggaggcg agaccctgta tgtcactgca gacctcaccc
3481tggaggacaa ccggcgggtg attgtcatgg agaagggacc ccttcccagc cccactgcag
3541ggctggagga gagcagtggc cagggaccaa gctcaccggt ggccctgctg gggcaggttc
3601aggacttcca gcagtctgca gagtgccagc cgaaggaaga gggttccagg gacccggcag
3661acccgagcca gcagggcaga gtgagaaacc ttagagagaa gttccaggcc ttgaactctg
3721tcggtttgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
3781gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
3841acctctggat ta

Download FASTA (ORF) (Full)