Construct: ORF ccsbBroad304_11923
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001162.1_s304c1, BRDN0000409536
- Derived from:
- ccsbBroadEn_11923
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PODXL2 (50512)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11923
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50512 | PODXL2 | podocalyxin like 2 | NM_015720.4 | 87.4% | 87.4% | 1003_1230del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1653
- ORF length:
- 1587
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ccggctgctg cgggccgccc ggctgccgcc gctgctttcg ccgctgctgc 121 ttctgctggt tgggggagcg ttcctgggtg cctgtgtggc tgggtctgat gagcctggcc 181 cagagggcct cacctccacc tccctgctag acctcctgct gcccactggc ttggagccac 241 tggactcaga ggagcctagt gagaccatgg gcctgggagc tgggctggga gcccctggct 301 caggcttccc cagcgaagag aatgaagagt ctcggattct gcagccacca cagtacttct 361 gggaagagga ggaagagctg aatgactcaa gtctggacct gggacccact gcagattatg 421 tttttcctga cttaactgag aaggcaggtt ccattgaaga caccagccag gctcaagagc 481 tgccaaacct cccctctccc ttgcccaaga tgaatctggt tgagcctccc tggcatatgc 541 ctcccagaga ggaggaagaa gaggaagagg aagaggagga gagggagaag gaagaggtag 601 agaaacaaga ggaggaggaa gaggaggagc tgctccctgt gaatggatcc caagaagaag 661 ccaagcctca ggtccgtgac ttttctctca ccagcagcag ccagacccca ggggccacca 721 aaagcaggca tgaagactcc ggggaccagg cctcatcagg tgtggaggtg gagagcagca 781 tggggcccag cttgctgctg ccttcagtca ccccaactac agtgactccg ggggaccagg 841 actccaccag ccaagaggca gaggccacag tgctgccagc tgcagggctt ggggtagagt 901 tcgaggctcc tcaggaagca agcgaggaag ccactgcagg agcagctggt ttgtctggcc 961 agcacgagga ggtgccggcc ttgccttcat tccctcaaac cacagctccc agtggggccg 1021 agcacccaga tgaagatccc cttggctcta gaacctcagc ctcttcccca cagctcctgg 1081 ccctggtgga agaggtgctg ccccgccatg gcagtggcca ccatggggcc tggcacatct 1141 ctctgagcaa gcccagcgag aaggagcagc accttctcat gacactggtg ggcgagcagg 1201 gggtggtgcc cactcaagat gtcctttcca tgctgggtga catccgcagg agcctggagg 1261 agattggcat ccagaactat tccacaacca gcagctgcca ggcgcgggcc agccaggtgc 1321 gcagcgacta cggcacgctc ttcgtggtgc tggtggtcat tggggccatc tgcatcatca 1381 tcattgcgct tggcctgctc tacaactgct ggcagcgccg gctgcccaag ctcaagcacg 1441 tgtcgcacgg cgaggagctg cgcttcgtgg agaacggctg ccacgacaac cccacgctgg 1501 acgtggccag cgacagccag tcggagatgc aggagaagca ccccagcctg aacggcggcg 1561 gggccctcaa cggcccgggg agctgggggg cgctcatggg gggcaagcgg gaccccgagg 1621 actcggacgt gttcgaggag gacacgcacc tgtgcccaac tttcttgtac aaagtggttg 1681 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 1741 accggtggcg cgttaagtcg acaatcaacc tctggatta