Construct: ORF ccsbBroad304_11987

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009726.1_s304c1, BRDN0000398952
Derived from:
ccsbBroadEn_11987
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LCMT1 (51451)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11987
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51451 LCMT1 leucine carboxyl methyltran... XM_011545864.1 99.9% 100% 24G>C
2 human 51451 LCMT1 leucine carboxyl methyltran... NM_016309.3 89.2% 80% (many diffs)
3 human 51451 LCMT1 leucine carboxyl methyltran... XM_011545862.2 84.5% 84.5% 24G>C;472_473ins165
4 human 51451 LCMT1 leucine carboxyl methyltran... NM_001032391.2 74% 65.5% (many diffs)
5 human 51451 LCMT1 leucine carboxyl methyltran... XM_005255354.4 65.5% 65.5% 0_1ins369
6 mouse 30949 Lcmt1 leucine carboxyl methyltran... XM_006507917.3 43% 45.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1137
ORF length:
1071
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct tccctgtgca agagaactcc catcagcagc gcccgacagc actctcctcc
121tcactgccca acactgggtg gcaaatcttt tcatcttggc cactctgata ggctggacag
181tgaggtcctt cttcctaggg cgcatggaaa cctgttgcag tcacctgggt ttgcggagtg
241ggaggaggtt tgcagtaagc attggctact ggcatgaccc ttacatacag cactttgtga
301gactgtctaa agagaggaaa gcccctgaaa tcaacagagg atattttgct cgagtccatg
361gtgtcagtca gcttataaag gcatttctac ggaagacaga atgtcattgt caaattgtca
421accttggggc aggcatggat accaccttct ggagattaaa ggatgaagat cttctcccaa
481gtaaatattt tgaggttgac tttccaatga ttgtcacgag aaagctgcac agtatcaaat
541gcaagcctcc cctatccagc cccattctag aactgcattc agaggacaca cttcagatgg
601atggacacat actggattca aagagatatg ccgttattgg agcagatctc cgagacctgt
661ctgaactgga agagaagcta aagaaatgta acatgaatac acaattgcca acactcctga
721tagctgaatg tgtgctggtt tacatgactc cagagcagtc cgcaaacctc ctgaagtggg
781cagccaacag ttttgagaga gccatgttca taaactacga acaggtgaac atgggtgatc
841ggtttgggca gatcatgatt gaaaacctgc ggagacgcca gtgtgacctg gcgggagtgg
901agacctgcaa gtcattagag tcacagaaag aacggctcct gtcgaatggg tgggaaacag
961catcggccgt cgacatgatg gagttgtaca acaggttacc tcgagctgaa gtgagcagga
1021tagaatcact tgaattcctg gatgaaatgg agctgctgga gcagctcatg cggcattact
1081gcctttgctg ggcaaccaaa ggaggaaatg agcttgggct gaaggagata acttattacc
1141caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
1201ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
1261tta

Download FASTA (ORF) (Full)

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