Construct: ORF ccsbBroad304_12001
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005366.1_s304c1, BRDN0000407299
- Derived from:
- ccsbBroadEn_12001
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DPH5 (51611)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12001
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51611 | DPH5 | diphthamide biosynthesis 5 | XM_005270939.5 | 26.7% | 26.7% | 1_378del |
| 2 | human | 51611 | DPH5 | diphthamide biosynthesis 5 | XM_011541570.3 | 26.7% | 26.7% | 1_378del |
| 3 | human | 51611 | DPH5 | diphthamide biosynthesis 5 | XM_017001461.2 | 26.7% | 26.7% | 1_378del |
| 4 | human | 51611 | DPH5 | diphthamide biosynthesis 5 | NM_001077395.2 | 16.1% | 16.1% | 1_714del |
| 5 | human | 51611 | DPH5 | diphthamide biosynthesis 5 | NM_001077394.1 | 16.1% | 16.1% | 1_717del |
| 6 | human | 51611 | DPH5 | diphthamide biosynthesis 5 | NM_015958.3 | 16.1% | 16.1% | 1_717del |
| 7 | human | 51611 | DPH5 | diphthamide biosynthesis 5 | XM_017001460.2 | 16.1% | 16.1% | 1_717del |
| 8 | human | 51611 | DPH5 | diphthamide biosynthesis 5 | XM_005270938.2 | 14.2% | 1.8% | 1_613del;720_721ins31 |
| 9 | human | 51611 | DPH5 | diphthamide biosynthesis 5 | XM_024447600.1 | 14.2% | 1.8% | 1_613del;720_721ins31 |
| 10 | human | 51611 | DPH5 | diphthamide biosynthesis 5 | XM_024447601.1 | 14.2% | 1.8% | 1_613del;720_721ins31 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 204
- ORF length:
- 138
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtg cactgtggac ttgggagaac cattgcattc cttgatcatc acaggaggca 121 gcatacatcc aatggagatg gagatgctaa gtctgttttc cataccagaa aatagctcag 181 aatctcaaag catcaatgga ctttgcccaa ctttcttgta caaagtggtt ggtaagccta 241 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 301 gcgttaagtc gacaatcaac ctctggatta