Construct: ORF ccsbBroad304_12035
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006896.1_s304c1, BRDN0000404587
- Derived from:
- ccsbBroadEn_12035
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SIAE (54414)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12035
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54414 | SIAE | sialic acid acetylesterase | NM_170601.5 | 33.4% | 24.6% | (many diffs) |
| 2 | human | 54414 | SIAE | sialic acid acetylesterase | NM_001199922.1 | 26.8% | 18.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 633
- ORF length:
- 567
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt cgcgccgggg cttgtactcg ggctggtgct gccattaatc ctgtgggccg 121 acagaagtgc aggtattggt tttcgctttg cttcatacat caataatgat atggtgctgc 181 agaaggagcc tgctggggca gtgatatggg gcttcggtac acctggagcc acagtgaccg 241 tgaccctgcg ccaaggtcag gaaaccatca tgaagaaagt gaccagtgtg aaagctcact 301 ctgatacgtg gatggtggta ctggatccta tgaagcctgg aggacctttc gaagtgatgg 361 cacaacagac tttggagaaa ataaacttca ccctgagagt tcatgacgtc ctgtttggag 421 atgtctggct ctgtagtggg cagagtaaca tgcagatgac tgtgttacag gtaatttgca 481 gagtctcagc aataatacac agtgttggtg atgaagaagg aaaagaagga ggaataaggg 541 ggcaacaagg agaagcaggg ctgtttctat ttagtgagta ccatgagcca agatatgttc 601 taaatgcttt gaatgaattc attaatttga cttacccaac tttcttgtac aaagtggttg 661 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 721 accggtggcg cgttaagtcg acaatcaacc tctggatta