Construct: ORF ccsbBroad304_12036
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000868.1_s304c1, BRDN0000405726
- Derived from:
- ccsbBroadEn_12036
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAJC10 (54431)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12036
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54431 | DNAJC10 | DnaJ heat shock protein fam... | NM_001271581.3 | 10.3% | 10.4% | 1_2007del;2103A>G |
| 2 | human | 54431 | DNAJC10 | DnaJ heat shock protein fam... | NM_018981.4 | 9.7% | 9.8% | 1_2145del;2241A>G |
| 3 | human | 54431 | DNAJC10 | DnaJ heat shock protein fam... | NR_073367.2 | 1.1% | 1_2468del;2564A>G;2703_20037del | |
| 4 | human | 54431 | DNAJC10 | DnaJ heat shock protein fam... | NR_073365.2 | 1.1% | 1_3329del;3425A>G;3564_20898del | |
| 5 | human | 54431 | DNAJC10 | DnaJ heat shock protein fam... | NR_073366.2 | 1.1% | 1_3432del;3528A>G;3667_21001del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 300
- ORF length:
- 234
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat taaaggaaaa gtgaaagctg gaaaagtaga ctgtcaggct tatgctcaga 121 catgccagaa agctgggatc agggcctatc caactgttaa gttttatttc tacgaaagag 181 caaagagaaa ttttcaagaa gagcagataa ataccagaga tgcaaaagca atcgctgcct 241 taataagtga aaaattggaa actctccgaa atcaaggcaa gaggaataag gatgaacttt 301 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 361 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 421 ggatta