Transcript: Human NM_001271581.3

Homo sapiens DnaJ heat shock protein family (Hsp40) member C10 (DNAJC10), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
DNAJC10 (54431)
Length:
20006
CDS:
431..2674

Additional Resources:

NCBI RefSeq record:
NM_001271581.3
NBCI Gene record:
DNAJC10 (54431)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001271581.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000062332 GCACCAGACATCTGTAGTAAT pLKO.1 1508 CDS 100% 13.200 18.480 N DNAJC10 n/a
2 TRCN0000299406 GCACCAGACATCTGTAGTAAT pLKO_005 1508 CDS 100% 13.200 18.480 N DNAJC10 n/a
3 TRCN0000062328 CCGGACATTAACTGGACTGAT pLKO.1 2098 CDS 100% 4.950 6.930 N DNAJC10 n/a
4 TRCN0000062330 CGAGCTTTACTACCAGAGTTA pLKO.1 1742 CDS 100% 4.950 6.930 N DNAJC10 n/a
5 TRCN0000299485 CGAGCTTTACTACCAGAGTTA pLKO_005 1742 CDS 100% 4.950 6.930 N DNAJC10 n/a
6 TRCN0000062331 GCATTGAAGTTACATCCTGAT pLKO.1 605 CDS 100% 4.050 3.240 N DNAJC10 n/a
7 TRCN0000299486 GCATTGAAGTTACATCCTGAT pLKO_005 605 CDS 100% 4.050 3.240 N DNAJC10 n/a
8 TRCN0000062329 CCAGTATGAAAGCTGGAACTA pLKO.1 760 CDS 100% 4.950 3.465 N DNAJC10 n/a
9 TRCN0000299484 CCAGTATGAAAGCTGGAACTA pLKO_005 760 CDS 100% 4.950 3.465 N DNAJC10 n/a
10 TRCN0000149064 GCAGGTTTGTTACATAGGTAT pLKO.1 6560 3UTR 100% 4.950 2.475 Y GLIPR1L2 n/a
11 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 15020 3UTR 100% 5.625 2.813 Y KLHL30 n/a
12 TRCN0000166364 CACACACACACACACACACAA pLKO.1 19363 3UTR 100% 4.950 2.475 Y KAAG1 n/a
13 TRCN0000009295 GCAGGTTTGTTACATAGGTAA pLKO.1 6560 3UTR 100% 4.950 2.475 Y OR11A1 n/a
14 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 15020 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001271581.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15862 pDONR223 0% 94.1% 94% None 846_847ins138;1800T>G;2103A>G n/a
2 ccsbBroad304_15862 pLX_304 0% 94.1% 94% V5 846_847ins138;1800T>G;2103A>G n/a
3 TRCN0000465524 GCCATCGTCATACTCGTTGGTTCC pLX_317 14.9% 94.1% 94% V5 846_847ins138;1800T>G;2103A>G n/a
4 ccsbBroadEn_08373 pDONR223 100% 94% 94% None (many diffs) n/a
5 ccsbBroad304_08373 pLX_304 0% 94% 94% V5 (many diffs) n/a
6 TRCN0000470792 AACTTACCCTAGTGACAGCACTGC pLX_317 18% 94% 94% V5 (many diffs) n/a
7 ccsbBroadEn_12036 pDONR223 100% 10.3% 10.4% None 1_2007del;2103A>G n/a
8 ccsbBroad304_12036 pLX_304 0% 10.3% 10.4% V5 1_2007del;2103A>G n/a
9 TRCN0000469914 AGCAAGGGCTTCCCGTTAATATTT pLX_317 100% 10.3% 10.4% V5 1_2007del;2103A>G n/a
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