Construct: ORF ccsbBroad304_12132

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002772.1_s304c1, BRDN0000407665
Derived from:
ccsbBroadEn_12132
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HERC6 (55008)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12132
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55008 HERC6 HECT and RLD domain contain... XM_017008333.1 61.7% 61.7% 1_705del
2 human 55008 HERC6 HECT and RLD domain contain... NM_001165136.1 38.3% 38.2% 1_1818del;1820_1821delACinsTG;1824_1826delCCT
3 human 55008 HERC6 HECT and RLD domain contain... XM_011532053.3 37.9% 37.7% 1_1854del;1856_1857delACinsTG;1860_1862delCCT
4 human 55008 HERC6 HECT and RLD domain contain... NM_017912.3 37% 37% 1_1929del
5 human 55008 HERC6 HECT and RLD domain contain... XM_005263083.4 36.6% 36.6% 1_1965del
6 human 55008 HERC6 HECT and RLD domain contain... XR_001741267.2 28.2% 1_2247del;3385_4024del
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1203
ORF length:
1137
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgca gatgtcagaa aagaaagcat acatgcttat gcatgaaaca attctgcaaa
121aaaaggatga atttcctcca tcacccagat ttatacttag agtcagacga agtcgcctgg
181ttaaagatgc tctgcgtcaa ttaagtcaag ctgaagctac tgacttctgc aaagtattag
241tggttgaatt tattaatgaa atttgtcctg agtctggagg ggttagttca gagttcttcc
301actgtatgtt tgaagagatg accaagccag aatatggaat gttcatgtat cctgaaatgg
361gttcctgcat gtggtttcct gccaagccta aacctgagaa gaaaagatat ttcctctttg
421gaatgctgtg tggactctcc ttattcaatt taaatgttgc taaccttcct ttcccactgg
481ctctgtataa aaaacttctg gaccaaaagc catcattgga agatttaaaa gaactcagtc
541ctcggttggg gaagagtttg caagaagttc tagatgatgc tgctgatgac attggagatg
601cgctctgcat acgcttttct atacactggg accaaaatga tgttgactta attccaaatg
661ggatctccat acctgtggac caaaccaaca agagagacta tgtttctaag tatattgatt
721acattttcaa cgtctctgta aaagcagttt atgaggaatt tcagagagga ttttatagag
781tctgtgagaa ggagatactt agacatttct accctgaaga actaatgaca gcaatcattg
841gaaatactga ttatgactgg aaacagtttg aacagaattc aaagtatgag caaggatacc
901aaaaatcaca tcctactata cagttgtttt ggaaggcttt ccacaaacta accttggatg
961aaaagaaaaa attcctcttt ttccttacag gacgtgatag gctgcatgca agaggcatac
1021agaaaatgga aatagtattt cgctgtcctg aaactttcag tgaaagagat cacccaacat
1081caataacttg tcataatatt ctctccctcc ctaagtattc tacaatggaa agaatggagg
1141aagcacttca agtagccatc aacaacaaca gaggatttgt ctcacccatg ctcacacagt
1201catacccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
1261tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
1321tctggatta

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