Construct: ORF ccsbBroad304_12151
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015654.1_s304c1, BRDN0000410205
- Derived from:
- ccsbBroadEn_12151
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF31 (55072)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12151
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55072 | RNF31 | ring finger protein 31 | NM_001310332.1 | 63.9% | 63.9% | 1_996del |
| 2 | human | 55072 | RNF31 | ring finger protein 31 | NM_017999.5 | 54.9% | 54.9% | 1_1449del |
| 3 | mouse | 268749 | Rnf31 | ring finger protein 31 | NM_194346.2 | 48.7% | 51% | (many diffs) |
| 4 | mouse | 268749 | Rnf31 | ring finger protein 31 | XM_006519062.2 | 48.5% | 50.8% | (many diffs) |
| 5 | mouse | 268749 | Rnf31 | ring finger protein 31 | XR_383184.1 | 42.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1833
- ORF length:
- 1767
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcg ggaagaaggc ctccagctag tgagcatgat ccgggaaggg gaagccgcag 121 gtgcctgtcc agaggagatc ttctcggctc tgcagtactc gggcactgag gtgcctctgc 181 agtggttgcg ctcagaactg ccctacgtcc tggagatggt ggctgagctg gctggacagc 241 aggaccctgg gctgggtgcc ttttcctgtc aggaggcccg gagagcctgg ctggatcgtc 301 atggcaacct tgatgaagct gtggaggagt gtgtgaggac caggcgaagg aaggtgcagg 361 agctccagtc tctaggcttt gggcctgagg aggggtctct ccaggcattg ttccagcacg 421 gaggtgatgt gtcacgggcc ctgactgagc tacagcgcca acgcctagag cccttccgcc 481 agcgcctctg ggacagtggc cctgagccca ccccttcctg ggatgggcca gacaagcaga 541 gcctggtcag gcggcttttg gcagtctacg cactccccag ctggggccgg gcagagctgg 601 cactgtcact gctgcaggag acacccagga actatgagtt gggggatgtg gtagaagctg 661 tgaggcacag ccaggaccgg gccttcctgc gccgcttgct tgcccaggag tgtgccgtgt 721 gtggctgggc cctgccccac aaccggatgc aggccctgac ttcctgtgag tgcaccatct 781 gtcctgactg cttccgccag cacttcacca tcgccttgaa ggagaagcac atcacagaca 841 tggtgtgccc tgcctgtggc cgccccgacc tcaccgatga cacacagttg ctcagctact 901 tctctaccct tgacatccag cttcgcgaga gcctagagcc agatgcctat gcgttgttcc 961 ataagaagct gaccgagggt gtgctgatgc gggaccccaa gttcttgtgg tgtgcccagt 1021 gctcctttgg cttcatatat gagcgtgagc agctggaggc aacttgtccc cagtgtcacc 1081 agaccttctg tgtgcgctgc aagcgccagt gggaggagca gcaccgaggt cggagctgtg 1141 aggacttcca gaactggaaa cgcatgaacg acccagaata ccaggcccag ggcctagcaa 1201 tgtatcttca ggaaaacggc attgactgcc ccaaatgcaa gttctcgtac gccctggccc 1261 gaggaggctg catgcacttt cactgtaccc agtgccgcca ccagttctgc agcggctgct 1321 acaatgcctt ttacgccaag aataaatgtc cagagcctaa ctgcagggtg aaaaagtccc 1381 tgcacggcca ccaccctcga gactgcctct tctacctgcg ggactggact gctctccggc 1441 ttcagaagct gctacaggac aataacgtca tgtttaatac agagcctcca gctggggccc 1501 gggcagtccc tggaggcggc tgccgagtga tagagcagaa ggaggttccc aatgggctca 1561 gggacgaagc ttgtggcaag gaaactccag ctggctatgc cggcctgtgc caggcacact 1621 acaaagagta tcttgtgagc ctcatcaatg cccactcgct ggacccagcc accttgtatg 1681 aggtggaaga gctggagacg gccactgagc gctacctgca cgtacgcccc cagcctttgg 1741 ctggagagga tccccctgct taccaggccc gcttgttaca gaagctgaca gaagaggtac 1801 ccttgggaca gagtatcccc cgcaggcgga agtacccaac tttcttgtac aaagtggttg 1861 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 1921 accggtggcg cgttaagtcg acaatcaacc tctggatta