Construct: ORF ccsbBroad304_12223

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001031.1_s304c1, BRDN0000404769
Derived from:
ccsbBroadEn_12223
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CDCA7L (55536)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12223
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55536 CDCA7L cell division cycle associa... NM_018719.5 99.7% 99.7% 163_165delCAG
2 human 55536 CDCA7L cell division cycle associa... NM_001127370.3 92.2% 92.2% 0_1ins102;61_63delCAG
3 human 55536 CDCA7L cell division cycle associa... NM_001127371.3 90% 90% 164_165ins135
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1425
ORF length:
1359
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gttggcgact cgctaccaga tccctaaaga agtggctgac atctttaacg
121cccccagtga tgatgaagag tttgttggct tccgagatga tgttcccatg gaaaccctct
181cgtcagagga gagctgcgat agttttgact cactagagtc agggaaacag gatgtgcgct
241ttcattccaa atacttcaca gaagagctaa gaagaatttt tatagaggac actgactcag
301agactgagga ttttgcagga tttacgcaga gtgatctgaa tggaaagact aacccagaag
361taatggtcgt ggagtcagat ttgagtgatg atggcaaagc atctttggtg agcgaggaag
421aggaagatga agaagaagat aaggctaccc ctagaagaag caggtctaga agaagtagta
481ttggtcttcg agtagccttt cagttcccca ccaagaagct ggccaacaaa ccagataaaa
541acagttcttc cgagcagttg ttttctagcg cacgcttaca gaatgagaaa aaaacaattc
601ttgaaagaaa gaaagactgt agacaggtga tacaaaggga agattctacc tctgagtctg
661aggatgactc tcgggatgag agccaggaga gttcagatgc tttgctgaaa aggaccatga
721acatcaagga gaacaaagcc atgcttgccc agttattggc ggaattgaac tcgatgccag
781atttcttccc agtacgaacc ccaacctcag cttctaggaa gaagacagtg aggcgggcct
841tctcggaggg acagatcacg cggcgtatga acccaacccg gagtgcgcgg cctcctgaga
901agtttgctct agagaacttc actgtctcag ccgctaaatt tgcggaagag ttttacagct
961tccgaagaag gaagacaatt ggggggaaat gccgggagta cagacgacgt caccgtatat
1021cttcttttcg gccagtggag gatatcaccg aagaggactt agaaaatgtt gccataactg
1081ttcgagataa aatctatgat aaagttctgg gtaacacgtg ccatcagtgt cgacaaaaga
1141ccatcgacac caagacagtg tgtcggaacc agggttgctg tggtgtgcga ggacagttct
1201gtggaccatg cctgcggaac cgctatgggg aggatgtcag atcggcattg ctggacccgg
1261attgggtgtg tcccccctgt cgtgggatct gcaattgcag ctactgtcgg aagcgtgacg
1321gccgctgtgc cacaggaatc ctcattcatc tggccaagtt ttatggttat gacaatgtta
1381aggaatatct ggagagctta caaaaggagc tggtagaaga caattaccca actttcttgt
1441acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
1501gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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