Construct: ORF ccsbBroad304_12230

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010144.1_s304c1, BRDN0000404014
Derived from:
ccsbBroadEn_12230
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DDX60 (55601)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12230
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55601 DDX60 DExD/H-box helicase 60 NM_017631.6 10.6% 10.6% 1_4587del
2 human 55601 DDX60 DExD/H-box helicase 60 XM_024454132.1 10.6% 10.6% 1_4587del
3 human 55601 DDX60 DExD/H-box helicase 60 XM_024454133.1 10.6% 10.6% 1_4587del
4 human 55601 DDX60 DExD/H-box helicase 60 XM_011532103.3 8.8% 8.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
615
ORF length:
549
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa aattatggag gactttacca ctttcctacg aattgtttcc aaactggctg
121atatgaatca ggaatatcaa ctcccattgt caaaaatcaa attcacaggt aaagaatgtg
181aagactctca actcgtatct catttgatga gctgcaagga aggaagagta gcaatttcac
241catttgtttg tctgtctggg aactttgatg atgatttgct tcgactagaa actccaaacc
301atgttactct aggcacaatc ggtgtcaatc gctctcaggc tccagtgctg ttgtcacaga
361aatttgataa ccgaggaagg aaaatgtcgc ttaatgccta tgcactggat ttctacaaac
421atggttcctt gataggatta gtccaggata acaggatgaa tgaaggagat gcttattatt
481tgttgaagga ttttgcactc accattaaat ctatcagtgt ttccttgcgt gagctatgtg
541aaaatgaaga cgacaacgtt gtcttagcct ttgaacaact gagtacaact ttttgggaaa
601agttaaacaa agtctaccca actttcttgt acaaagtggt tggtaagcct atccctaacc
661ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
721cgacaatcaa cctctggatt a

Download FASTA (ORF) (Full)