Construct: ORF ccsbBroad304_12260
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018472.1_s304c1, BRDN0000406877
- Derived from:
- ccsbBroadEn_12260
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSR1 (55720)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12260
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55720 | TSR1 | TSR1 ribosome maturation fa... | NM_018128.5 | 81.5% | 81.4% | 1_444del;1156A>G |
| 2 | mouse | 104662 | Tsr1 | TSR1 20S rRNA accumulation | NM_177325.3 | 69.4% | 71.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2034
- ORF length:
- 1968
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc taaagtagct gataccatcc tgttcctcct tgatccacta gaaggctggg 121 acagcaccgg tgattactgt ctttcctgcc tctttgctca gggccttccg acctatacac 181 tagctgtcca ggggatttct ggcctcccac tgaagaaaca aatagatacc aggaagaagc 241 taagtaaagc agtggagaag cgctttccgc atgacaaact cctcttgtta gacactcaac 301 aggaggcagg gatgctgctt aggcagttgg ctaaccagaa gcaacagcat cttgcttttc 361 gagatcggcg ggcctaccta tttgcccatg ctgttgattt tgttcctagt gaagagaata 421 acttggtggg caccttgaaa atttcaggct atgttcgagg gcagactctg aatgtcaata 481 ggttgctgca tatcgttgga tatggtgatt tccagatgaa acagatagat gcccccggag 541 accctttccc tttaaatcct agaggaatta aaccccaaaa ggacccagac atggcaatgg 601 agatttgtgc tacggatgct gtagatgata tggaagaagg tcttaaagtc ctaatgaagg 661 cagaccctgg tagacaggaa tccttgcaag cagaggttat cccagatcca atggagggag 721 agcaaacctg gcccactgag gaggagctga gcgaggcaaa ggatttcttg aaggaaggtt 781 ctaaggtggt aaagaaggtc cccaaaggaa catccagtta ccaagctgaa tggattttgg 841 atggtggcag ccaaagtggt ggggaaggag atgaatatga atatgatgat atggaacatg 901 aggattttat ggaggaggaa tctcaggatg agagtagtga agaagaggaa gaatatgaaa 961 ctatgactat tggggagtct gtgcatgatg atctgtatga taagaaagta gatgaagaag 1021 ctgaggcaaa aatgttggag aaatataaac aagaaagact ggaagagatg tttccagatg 1081 aagtggacac gccccgtgat gtggctgctc gaattcgatt tcagaaatac agaggcctta 1141 agagcttccg gacatctcca tgggatccta aggaaaacct tcctcaagat tatgctcgaa 1201 tatttcagtt tcagaacttt actaacacta ggaaaagcat ctttaaagag gttgaagaaa 1261 aagaggttga aggagctgag gttggctggt atgtcacact tcatgtctct gaagtccccg 1321 tctcagtggt cgagtgcttc aggcaaggaa cacccttgat tgcattttct ttactacctc 1381 atgaacagaa gatgtcagta ttgaatatgg tggtgaggcg tgaccctggc aacactgaac 1441 ctgtgaaagc caaggaagag ctcatatttc actgtggatt caggcgcttc cgagcctcac 1501 ctttattctc tcagcacact gcagcggaca aacataaatt gcagagattc ctgactgctg 1561 acatggccct ggtggcgaca gtctatgcgc caatcacttt tcctcctgca tctgtgctgc 1621 ttttcaagca aaaaagcaat ggaatgcaca gcctcattgc tacaggccat cttatgtcag 1681 tagatccaga cagaatggtc atcaagagag ttgttctgag tggtcatcct ttcaaaattt 1741 ttactaagat ggcagtagta cgttacatgt tcttcaacag agaggatgtg ctgtggttta 1801 aaccagtgga actgagaacg aagtggggcc ggagaggaca tatcaaggaa cctttaggta 1861 cccatggcca catgaaatgc agctttgatg ggaagctaaa atctcaagac acagtactga 1921 tgaacctgta taaacgagtc ttccccaaat ggacttatga tccatatgta ccagaaccag 1981 taccctggct gaaaagtgag atttcttcaa cagtgcctca agggggcatg gagtacccaa 2041 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 2101 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta