Construct: ORF ccsbBroad304_12265
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016489.1_s304c1, BRDN0000402735
- Derived from:
- ccsbBroadEn_12265
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ENAH (55740)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12265
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55740 | ENAH | ENAH actin regulator | NM_018212.6 | 23.3% | 22.1% | (many diffs) |
| 2 | human | 55740 | ENAH | ENAH actin regulator | NM_001008493.3 | 22.5% | 21.3% | (many diffs) |
| 3 | human | 55740 | ENAH | ENAH actin regulator | XM_024448316.1 | 17.1% | 15.5% | (many diffs) |
| 4 | human | 55740 | ENAH | ENAH actin regulator | XM_024448317.1 | 17.1% | 15.5% | (many diffs) |
| 5 | human | 55740 | ENAH | ENAH actin regulator | XM_024448315.1 | 16.9% | 15.2% | (many diffs) |
| 6 | human | 55740 | ENAH | ENAH actin regulator | XM_024448314.1 | 16.7% | 15.1% | (many diffs) |
| 7 | human | 55740 | ENAH | ENAH actin regulator | XM_024448313.1 | 16.6% | 15% | (many diffs) |
| 8 | human | 55740 | ENAH | ENAH actin regulator | XM_024448311.1 | 16.4% | 14.8% | (many diffs) |
| 9 | human | 55740 | ENAH | ENAH actin regulator | XM_017001752.1 | 16.2% | 14.9% | (many diffs) |
| 10 | human | 55740 | ENAH | ENAH actin regulator | XM_024448309.1 | 16.1% | 14.6% | (many diffs) |
| 11 | human | 55740 | ENAH | ENAH actin regulator | XM_024448310.1 | 16.1% | 14.6% | (many diffs) |
| 12 | human | 55740 | ENAH | ENAH actin regulator | XM_017001751.1 | 16% | 15.2% | (many diffs) |
| 13 | human | 55740 | ENAH | ENAH actin regulator | XM_017001749.1 | 15.7% | 14.8% | (many diffs) |
| 14 | human | 55740 | ENAH | ENAH actin regulator | XM_017001748.1 | 15.6% | 14.8% | (many diffs) |
| 15 | human | 55740 | ENAH | ENAH actin regulator | XM_017001747.1 | 15.6% | 14.8% | (many diffs) |
| 16 | human | 55740 | ENAH | ENAH actin regulator | XM_017001750.1 | 15.4% | 14.2% | (many diffs) |
| 17 | human | 55740 | ENAH | ENAH actin regulator | XM_011544229.2 | 15.3% | 14.5% | (many diffs) |
| 18 | human | 55740 | ENAH | ENAH actin regulator | XM_017001746.2 | 15.2% | 14.4% | (many diffs) |
| 19 | human | 55740 | ENAH | ENAH actin regulator | XM_024448319.1 | 13% | 11.8% | (many diffs) |
| 20 | human | 55740 | ENAH | ENAH actin regulator | XM_024448318.1 | 12.8% | 11.6% | (many diffs) |
| 21 | human | 55740 | ENAH | ENAH actin regulator | XM_024448308.1 | 12.8% | 11.6% | (many diffs) |
| 22 | human | 55740 | ENAH | ENAH actin regulator | XM_024448307.1 | 12.7% | 11.5% | (many diffs) |
| 23 | human | 55740 | ENAH | ENAH actin regulator | XM_024448306.1 | 12.5% | 11.4% | (many diffs) |
| 24 | human | 55740 | ENAH | ENAH actin regulator | XM_024448305.1 | 12.5% | 11.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 504
- ORF length:
- 438
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga aattcaaaga agacaactac aagaacagca acggcaaaag gagctggagc 121 gggaaaggct ggagcgagaa agaatggaaa gagaaaggtt ggagagagag aggttagaaa 181 gggaaaggct ggagagggag cgactggaac aagaacagct ggagagagag agacaagaac 241 gggaacggca ggaacgcctg gagcggcagg aacgcctgga gcggcaggaa cgcctggatc 301 gggagaggca agaaagacaa gaacgagaga ggctggagag actggaacgg gagaggcaag 361 aaagggagcg acaagagcag ttagaaaggg aacagctgga atgggagaga gagcgcagaa 421 tatcaagtgc tggtaagaag tttacaaact cttctgtgtt cttgtatctg agcttggatg 481 atgtctcttg tgcagtcatg tgttncccaa ctttcttgta caaagtggtt ggtaagccta 541 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 601 gcgttaagtc gacaatcaac ctctggatta