Construct: ORF ccsbBroad304_12294
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014779.1_s304c1, BRDN0000402603
- Derived from:
- ccsbBroadEn_12294
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BARX1 (56033)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12294
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56033 | BARX1 | BARX homeobox 1 | NM_021570.4 | 39.3% | 39.3% | 1_462del |
| 2 | mouse | 12022 | Barx1 | BarH-like homeobox 1 | NM_007526.4 | 35.1% | 37.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 366
- ORF length:
- 300
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg cctggagaaa cgcttcgaga agcagaagta cctttccacg ccggacagaa 121 tagatcttgc tgagtccctg ggcctgagcc agttgcaggt gaagacgtgg taccagaatc 181 ggaggatgaa gtggaagaaa atagtgctgc agggcggcgg cctggagtct cccaccaagc 241 ccaaggggcg gcccaagaag aactcaattc caacgagcga gcagcttact gagcaggagc 301 gcgccaagga tgcagagaaa ccggcggagg tgccgggcga gcccagcgac aggagccgcg 361 aggactgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 421 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 481 acctctggat ta