Construct: ORF ccsbBroad304_12308
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009393.1_s304c1, BRDN0000403659
- Derived from:
- ccsbBroadEn_12308
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBQLN4 (56893)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12308
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 56893 | UBQLN4 | ubiquilin 4 | NM_001304342.2 | 10.6% | 10.6% | 1_1557del |
2 | human | 56893 | UBQLN4 | ubiquilin 4 | NM_020131.5 | 10.3% | 10.3% | 1_1617del |
3 | human | 56893 | UBQLN4 | ubiquilin 4 | XR_921895.3 | 5.6% | 1_1413del;1600_3279del | |
4 | mouse | 94232 | Ubqln4 | ubiquilin 4 | XM_006502396.2 | 10.2% | 10.8% | (many diffs) |
5 | mouse | 94232 | Ubqln4 | ubiquilin 4 | NM_033526.2 | 9.3% | 9.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 252
- ORF length:
- 186
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat ccagcttttg gctggaagtg gaaactcaca ggtgcagacg ccagaagtga 121 gatttcagca gcagctggag cagctcaact ccatgggctt catcaatcgt gaggctaacc 181 tgcaggccct gattgccaca ggaggggaca tcaacgcagc tatcgagaga ctgctgggct 241 cccagctctc ctacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 301 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 361 caatcaacct ctggatta