Construct: ORF ccsbBroad304_12322
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010243.1_s304c1, BRDN0000407626
- Derived from:
- ccsbBroadEn_12322
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC12A9 (56996)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12322
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56996 | SLC12A9 | solute carrier family 12 me... | NM_001267814.2 | 99.9% | 100% | 987T>C |
| 2 | human | 56996 | SLC12A9 | solute carrier family 12 me... | NM_001267812.1 | 84.5% | 84.4% | (many diffs) |
| 3 | human | 56996 | SLC12A9 | solute carrier family 12 me... | XM_005250502.2 | 64.9% | 64.4% | (many diffs) |
| 4 | human | 56996 | SLC12A9 | solute carrier family 12 me... | XM_006716054.2 | 64.9% | 64.4% | (many diffs) |
| 5 | human | 56996 | SLC12A9 | solute carrier family 12 me... | NM_001363493.2 | 58.6% | 58.2% | (many diffs) |
| 6 | human | 56996 | SLC12A9 | solute carrier family 12 me... | NM_020246.4 | 58.6% | 58.2% | (many diffs) |
| 7 | human | 56996 | SLC12A9 | solute carrier family 12 me... | NM_001363494.1 | 57.9% | 57.2% | (many diffs) |
| 8 | human | 56996 | SLC12A9 | solute carrier family 12 me... | XM_006716055.3 | 57.8% | 53.9% | (many diffs) |
| 9 | human | 56996 | SLC12A9 | solute carrier family 12 me... | XM_011516414.1 | 52.4% | 52% | (many diffs) |
| 10 | human | 56996 | SLC12A9 | solute carrier family 12 me... | XM_005250504.4 | 32.3% | 31.8% | (many diffs) |
| 11 | mouse | 83704 | Slc12a9 | solute carrier family 12 (p... | NM_031406.3 | 50.7% | 54.3% | (many diffs) |
| 12 | mouse | 83704 | Slc12a9 | solute carrier family 12 (p... | XM_006504633.3 | 50.7% | 54.3% | (many diffs) |
| 13 | mouse | 83704 | Slc12a9 | solute carrier family 12 (p... | XR_001784760.1 | 42% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1680
- ORF length:
- 1614
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cagcgagagc tcacctctgc tggcctaccg gctcctgggg gaggaggggg 121 ttgccctccc tgccaatggg gccgggggtc ctggaggggc gtctgcccgg aagctgtcca 181 ccttcctggg tgtggtggtg cccactgtcc tgtccatgtt cagcatagtt gtttttctga 241 ggattgatgc cacagggccc agtgggctcc gggtcctgcc ccagggctac ggctggaacc 301 tgctgtatgg ctccctgctg ctgggccttg tgggtggggt ctgcaccctg ggagccggcc 361 tctatgcccg ggcctcattc ctcacattcc tgctggtctc tggctccctg gcctctgtgc 421 tcatcagttt tgtggctgtg gggccgaggg acatccgctt gactcctagg cctggcccca 481 atggctcctc cctgccgccc cggtttggcc acttcaccgg cttcaacagc agtaccctga 541 aggacaactt gggcgctggc tatgctgagg actacaccac gggagccgtg atgaattttg 601 ccagcgtctt tgctgtcctc tttaacggct gtacaggcat catggctggg gccaacatgt 661 caggggagct gaaggacccc agccgggcga tccctctggg cacgatcgtc gccgtcgcct 721 acaccttctt cgtctatgtc ctgcttttct ttctctccag cttcacttgt gacaggaccc 781 tgctgcagga agactatggg ttcttccgcg ccatcagcct gtggccccca ctggtgttga 841 tcggaatcta tgccacagcg ctctcagcgt ccatgagctc gctcattggt gcctcccgca 901 tcctccatgc cctggcccgg gatgacctct ttggcgtgat cttggcaccg gccaaggttg 961 tgtcccgagg gggaaacccc tgggcagctg tactttattc ttggggcctg gtgcagctgg 1021 tgctcctggc tgggaagctg aacacactgg ccgctgtggt cactgtcttc tacctggtgg 1081 cctatgctgc cgtggacctg tcctgcctga gcctggagtg ggcctcggcc cccaacttcc 1141 gccccacctt cagcctgttc tcctggcaca cctgcctgct gggggtggcc tcctgcctgc 1201 tcatgatgtt cctcatcagt cctggcgcgg ctggtggctc cctgctcctc atgggtctgc 1261 tggctgccct gctcaccgcg cgaggaggcc ccagtagctg gggctatgtc agccaggcct 1321 tgcttttcca ccaggtgcgt aagtatctgc ttcggctgga cgtccggaag gatcacgtga 1381 agttctggcg gccccagctg ctgctcctgg tggggaaccc ccggggcgcc ctgcctctgc 1441 tgcggttggc caaccagctt aagaaggggg ggctgtatgt gctgggccac gtcaccctgg 1501 gagacctcga ctccctgccc tcggaccctg tacagccgca gtatggggca tggctcagcc 1561 tggtggaccg tgcccaggtg aaggcttttg tggatctaac cctctcaccc tccgtgcgcc 1621 agggggctca gcatctgctg cgaatctccg gcctcgagtc aaactcgcat cccctgccgt 1681 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1741 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1801 ggatta