Construct: ORF ccsbBroad304_12333
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015483.1_s304c1, BRDN0000404563
- Derived from:
- ccsbBroadEn_12333
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DOLPP1 (57171)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12333
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57171 | DOLPP1 | dolichyldiphosphatase 1 | NM_001135917.2 | 49.7% | 49.7% | 1_294del |
| 2 | human | 57171 | DOLPP1 | dolichyldiphosphatase 1 | NM_020438.5 | 40.7% | 40.7% | 1_294del;461_589del |
| 3 | human | 57171 | DOLPP1 | dolichyldiphosphatase 1 | NR_072983.2 | 14.8% | 1_230del;522_1955del | |
| 4 | mouse | 57170 | Dolpp1 | dolichyl pyrophosphate phos... | NM_001290508.1 | 62.6% | 67.1% | (many diffs) |
| 5 | mouse | 57170 | Dolpp1 | dolichyl pyrophosphate phos... | XM_006498207.3 | 62.6% | 67.1% | (many diffs) |
| 6 | mouse | 57170 | Dolpp1 | dolichyl pyrophosphate phos... | XM_017319147.1 | 54.7% | 58.7% | (many diffs) |
| 7 | mouse | 57170 | Dolpp1 | dolichyl pyrophosphate phos... | NM_001290509.1 | 44.6% | 48.2% | (many diffs) |
| 8 | mouse | 57170 | Dolpp1 | dolichyl pyrophosphate phos... | NM_020329.4 | 36.8% | 39.4% | (many diffs) |
| 9 | mouse | 57170 | Dolpp1 | dolichyl pyrophosphate phos... | NR_110963.1 | 8.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 357
- ORF length:
- 291
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc ctccagccat tcccagttta tgtggttctt ctccgtctat tccttccttt 121 tcctgtattt aagaatgcac caaacaaaca acgccaggtt cctggacttg ctgtggaggc 181 acgtgctctc cctgggactc ctcgctgtgg ccttcctagt ctcctacagc aggcctgtct 241 ccgagttctt cctaatccga gacacaagcc tcattcccaa cgtactctgg tttgagtaca 301 cggtaacccg ggcagaagcc aggaacagac aacgcaagct ggggacgaaa ctgcagtgcc 361 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 421 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 481 tta