Construct: ORF ccsbBroad304_12405
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002655.1_s304c1, BRDN0000407273
- Derived from:
- ccsbBroadEn_12405
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLD4 (57804)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12405
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57804 | POLD4 | DNA polymerase delta 4, acc... | NM_001256870.1 | 42.1% | 41.7% | 99_105delGGAGGAGinsT;107C>T;109_237del |
| 2 | human | 57804 | POLD4 | DNA polymerase delta 4, acc... | NM_021173.5 | 31.1% | 30.8% | 99_105delGGAGGAGinsT;107C>T;109_321del |
| 3 | human | 57804 | POLD4 | DNA polymerase delta 4, acc... | NR_046411.1 | 5.7% | 1_197del;300_1760del | |
| 4 | mouse | 69745 | Pold4 | polymerase (DNA-directed), ... | NM_027196.4 | 28.9% | 28% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 168
- ORF length:
- 102
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ccggaagcgg ctcatcactg attcctaccc ggttgtgaag aggagggagg 121 ggcccgctgg gcacagcaag ggggagctgg cacccgagct aggtctctgc ccaactttct 181 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 241 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta