Construct: ORF ccsbBroad304_12433

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001851.1_s304c1, BRDN0000403601
Derived from:
ccsbBroadEn_12433
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
UBE2O (63893)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12433
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 63893 UBE2O ubiquitin conjugating enzym... XM_017024935.1 36.7% 36.6% 1_2403del;3066G>T;3544G>A
2 human 63893 UBE2O ubiquitin conjugating enzym... NM_022066.4 36% 35.9% 1_2478del;3141G>T;3619G>A
3 human 63893 UBE2O ubiquitin conjugating enzym... XM_005257567.1 35.9% 35.8% 1_2481del;3144G>T;3622G>A
4 human 63893 UBE2O ubiquitin conjugating enzym... XM_024450867.1 35.6% 35.5% 1_2514del;3177G>T;3655G>A
5 human 63893 UBE2O ubiquitin conjugating enzym... XM_024450866.1 35% 34.9% 1_2586del;3249G>T;3727G>A
6 human 63893 UBE2O ubiquitin conjugating enzym... XM_024450865.1 35% 34.9% 1_2589del;3252G>T;3730G>A
7 mouse 217342 Ube2o ubiquitin-conjugating enzym... XM_011248960.1 55.8% 59.4% (many diffs)
8 mouse 217342 Ube2o ubiquitin-conjugating enzym... XM_006533106.1 32.3% 34.4% (many diffs)
9 mouse 217342 Ube2o ubiquitin-conjugating enzym... NM_173755.3 31.7% 33.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1464
ORF length:
1398
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgac tgtggagcag ctgctgacgg gctcgcccac ctctccgact gtggagcctg
121agaagccaac tcgggagaag aagtttctgg atgacatcaa gaagctacag gaaaacctca
181agaagaccct ggacaatgtg gccattgtag aggaggagaa gatggaagca gtgcccgacg
241tagagcgcaa ggaggacaag cccgaggggc agtcacctgt gaaggctgag tggcccagcg
301aaaccccggt gctgtgccag cagtgtggcg gcaagcctgg cgtcaccttc accagcgcca
361agggcgaggt cttctccgta ctggagtttg caccctcaaa tcattctttt aagaaaattg
421agttccagcc tccagaagcc aagaagttct tcagcacagt gcggaaggag atggcgctgc
481tggctacctc actgcctgag ggcatcatgg tcaagacttt tgaagataga atggacctct
541tctcagctct catcaagggc cccactcgaa ccccctacga ggatggcctc tacttgtttg
601acatccagct ccccaacatc tacccagccg tgccccccca cttctgctac ctctcccaat
661gcagtggccg cctgaacccc aacctgtatg acaatgggaa ggtgtgtgtc agcctcctgg
721gcacctgtat tggaaagggg acagagaggt ggacaagcaa gtccagcctt ctccaggtgc
781tcatctccat ccaaggtctg atcctggtaa atgaaccata ctacaacgaa gccggcttcg
841acagtgaccg aggcctgcag gaaggctatg aaaacagtcg ctgttacaat gagatggcgc
901tgatccgcgt ggtgcagtcc atgacccagc tggtgcggcg gccccccgag gtctttgagc
961aggagatcag gcaacacttt agcactggtg gctggcggct ggtgaaccgt atcgagtcct
1021ggctggaaac ccatgccctg ctggagaagg cccaggcact gcccaacggg gtgcccaagg
1081ccagcagctc gccagagccc ccagctgtag ccgagctgtc agactccggc caacaagaac
1141ctgaggatgg agggccagcc ccaggagagg cctcccaggg ctcagactca gagggcggtg
1201cccagagcct ggcctcagct agcagggacc acacagacca gacttcggag accgcaccag
1261acgcatcggt gccacccagt gtgaaaccaa agaagcggag aaagagctac cggagcttct
1321tacctgagaa gagtggctac cctgacatcg gcttccccct cttcccactt tccaagggtt
1381tcatcaagag catccggggt gtcctgacgc agttccgggc tgccctgcta gaggcaggca
1441tgccggagtg cacagaggac aagtacccaa ctttcttgta caaagtggtt ggtaagccta
1501tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
1561gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)