Construct: ORF ccsbBroad304_12433
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001851.1_s304c1, BRDN0000403601
- Derived from:
- ccsbBroadEn_12433
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBE2O (63893)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12433
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 63893 | UBE2O | ubiquitin conjugating enzym... | XM_017024935.1 | 36.7% | 36.6% | 1_2403del;3066G>T;3544G>A |
| 2 | human | 63893 | UBE2O | ubiquitin conjugating enzym... | NM_022066.4 | 36% | 35.9% | 1_2478del;3141G>T;3619G>A |
| 3 | human | 63893 | UBE2O | ubiquitin conjugating enzym... | XM_005257567.1 | 35.9% | 35.8% | 1_2481del;3144G>T;3622G>A |
| 4 | human | 63893 | UBE2O | ubiquitin conjugating enzym... | XM_024450867.1 | 35.6% | 35.5% | 1_2514del;3177G>T;3655G>A |
| 5 | human | 63893 | UBE2O | ubiquitin conjugating enzym... | XM_024450866.1 | 35% | 34.9% | 1_2586del;3249G>T;3727G>A |
| 6 | human | 63893 | UBE2O | ubiquitin conjugating enzym... | XM_024450865.1 | 35% | 34.9% | 1_2589del;3252G>T;3730G>A |
| 7 | mouse | 217342 | Ube2o | ubiquitin-conjugating enzym... | XM_011248960.1 | 55.8% | 59.4% | (many diffs) |
| 8 | mouse | 217342 | Ube2o | ubiquitin-conjugating enzym... | XM_006533106.1 | 32.3% | 34.4% | (many diffs) |
| 9 | mouse | 217342 | Ube2o | ubiquitin-conjugating enzym... | NM_173755.3 | 31.7% | 33.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1464
- ORF length:
- 1398
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac tgtggagcag ctgctgacgg gctcgcccac ctctccgact gtggagcctg 121 agaagccaac tcgggagaag aagtttctgg atgacatcaa gaagctacag gaaaacctca 181 agaagaccct ggacaatgtg gccattgtag aggaggagaa gatggaagca gtgcccgacg 241 tagagcgcaa ggaggacaag cccgaggggc agtcacctgt gaaggctgag tggcccagcg 301 aaaccccggt gctgtgccag cagtgtggcg gcaagcctgg cgtcaccttc accagcgcca 361 agggcgaggt cttctccgta ctggagtttg caccctcaaa tcattctttt aagaaaattg 421 agttccagcc tccagaagcc aagaagttct tcagcacagt gcggaaggag atggcgctgc 481 tggctacctc actgcctgag ggcatcatgg tcaagacttt tgaagataga atggacctct 541 tctcagctct catcaagggc cccactcgaa ccccctacga ggatggcctc tacttgtttg 601 acatccagct ccccaacatc tacccagccg tgccccccca cttctgctac ctctcccaat 661 gcagtggccg cctgaacccc aacctgtatg acaatgggaa ggtgtgtgtc agcctcctgg 721 gcacctgtat tggaaagggg acagagaggt ggacaagcaa gtccagcctt ctccaggtgc 781 tcatctccat ccaaggtctg atcctggtaa atgaaccata ctacaacgaa gccggcttcg 841 acagtgaccg aggcctgcag gaaggctatg aaaacagtcg ctgttacaat gagatggcgc 901 tgatccgcgt ggtgcagtcc atgacccagc tggtgcggcg gccccccgag gtctttgagc 961 aggagatcag gcaacacttt agcactggtg gctggcggct ggtgaaccgt atcgagtcct 1021 ggctggaaac ccatgccctg ctggagaagg cccaggcact gcccaacggg gtgcccaagg 1081 ccagcagctc gccagagccc ccagctgtag ccgagctgtc agactccggc caacaagaac 1141 ctgaggatgg agggccagcc ccaggagagg cctcccaggg ctcagactca gagggcggtg 1201 cccagagcct ggcctcagct agcagggacc acacagacca gacttcggag accgcaccag 1261 acgcatcggt gccacccagt gtgaaaccaa agaagcggag aaagagctac cggagcttct 1321 tacctgagaa gagtggctac cctgacatcg gcttccccct cttcccactt tccaagggtt 1381 tcatcaagag catccggggt gtcctgacgc agttccgggc tgccctgcta gaggcaggca 1441 tgccggagtg cacagaggac aagtacccaa ctttcttgta caaagtggtt ggtaagccta 1501 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1561 gcgttaagtc gacaatcaac ctctggatta