Transcript: Human XM_024450867.1

PREDICTED: Homo sapiens ubiquitin conjugating enzyme E2 O (UBE2O), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
UBE2O (63893)
Length:
5419
CDS:
94..4008

Additional Resources:

NCBI RefSeq record:
XM_024450867.1
NBCI Gene record:
UBE2O (63893)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024450867.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000272906 TCGGAACCCTGTTGCCGATTT pLKO_005 4237 3UTR 100% 10.800 15.120 N UBE2O n/a
2 TRCN0000004589 CGGGTCTCTTCTTCGATGATT pLKO.1 950 CDS 100% 5.625 7.875 N UBE2O n/a
3 TRCN0000272907 CGGGTCTCTTCTTCGATGATT pLKO_005 950 CDS 100% 5.625 7.875 N UBE2O n/a
4 TRCN0000004587 CGATGATTCCTATGGCTTCTA pLKO.1 963 CDS 100% 4.950 3.960 N UBE2O n/a
5 TRCN0000272910 CGATGATTCCTATGGCTTCTA pLKO_005 963 CDS 100% 4.950 3.960 N UBE2O n/a
6 TRCN0000272909 ATGTGAGTGTTTACGACATTG pLKO_005 2042 CDS 100% 10.800 7.560 N UBE2O n/a
7 TRCN0000284903 TCGTCATCCGCATCGGCAATA pLKO_005 2099 CDS 100% 10.800 7.560 N UBE2O n/a
8 TRCN0000004590 TGTGTTGAGATGCTGCCGTTT pLKO.1 4167 3UTR 100% 4.050 2.835 N UBE2O n/a
9 TRCN0000010895 CAAGGGTTTCATCAAGAGCAT pLKO.1 3915 CDS 100% 2.640 1.848 N UBE2O n/a
10 TRCN0000004588 GACATCAAGAAGCTACAGGAA pLKO.1 2695 CDS 100% 2.640 1.584 N UBE2O n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024450867.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12434 pDONR223 100% 56.8% 56.8% None 1_1686del;2057_2059delAGC n/a
2 ccsbBroad304_12434 pLX_304 0% 56.8% 56.8% V5 1_1686del;2057_2059delAGC n/a
3 TRCN0000491784 CAAGTCACCGCTGTGGCTCATGAT pLX_317 16.4% 56.8% 56.8% V5 1_1686del;2057_2059delAGC n/a
4 ccsbBroadEn_12433 pDONR223 100% 35.6% 35.5% None 1_2514del;3177G>T;3655G>A n/a
5 ccsbBroad304_12433 pLX_304 0% 35.6% 35.5% V5 1_2514del;3177G>T;3655G>A n/a
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