Construct: ORF ccsbBroad304_12451
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004127.1_s304c1, BRDN0000404465
- Derived from:
- ccsbBroadEn_12451
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIF9 (64147)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12451
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64147 | KIF9 | kinesin family member 9 | XM_017007031.1 | 13.7% | 13.3% | (many diffs) |
| 2 | human | 64147 | KIF9 | kinesin family member 9 | XM_011534005.2 | 12.4% | 12.1% | (many diffs) |
| 3 | human | 64147 | KIF9 | kinesin family member 9 | XM_017007030.1 | 12.4% | 12.1% | (many diffs) |
| 4 | human | 64147 | KIF9 | kinesin family member 9 | NM_022342.5 | 12.3% | 12% | (many diffs) |
| 5 | human | 64147 | KIF9 | kinesin family member 9 | XM_006713291.3 | 11.9% | 11.6% | (many diffs) |
| 6 | human | 64147 | KIF9 | kinesin family member 9 | XM_005265389.4 | 11.7% | 11.4% | (many diffs) |
| 7 | human | 64147 | KIF9 | kinesin family member 9 | NM_001134878.2 | 11.3% | 11% | (many diffs) |
| 8 | human | 64147 | KIF9 | kinesin family member 9 | NM_182902.3 | 11.3% | 11% | (many diffs) |
| 9 | human | 64147 | KIF9 | kinesin family member 9 | XM_011534004.2 | 11.3% | 11% | (many diffs) |
| 10 | human | 64147 | KIF9 | kinesin family member 9 | XM_017007029.1 | 11.3% | 11% | (many diffs) |
| 11 | human | 64147 | KIF9 | kinesin family member 9 | XM_011534003.2 | 11.1% | 10.8% | (many diffs) |
| 12 | human | 64147 | KIF9 | kinesin family member 9 | XM_005265388.4 | 10.8% | 10.5% | (many diffs) |
| 13 | human | 64147 | KIF9 | kinesin family member 9 | XR_002959567.1 | 10.3% | (many diffs) | |
| 14 | human | 64147 | KIF9 | kinesin family member 9 | XR_940489.2 | 9.8% | (many diffs) | |
| 15 | human | 64147 | KIF9 | kinesin family member 9 | XR_940488.2 | 9.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 339
- ORF length:
- 273
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg tactaggaaa aaagttcatg catttgtccg tgtcaaaccc accgatgact 121 ttgctcatga aatgatcaga tacggagatg acaaaagaag cattgatatt cacttaaaaa 181 aagacattcg gagaggagtt gtcaataacc aacagacaga ctggtcgttt aagttggatg 241 gagttcttca cgatgcctcc caggacttgg tttatgagac agttgcaaag gatgtggttt 301 ctcaggccct cgatggctat aatggtaact taattaatta cccaactttc ttgtacaaag 361 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 421 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta