Construct: ORF ccsbBroad304_12453

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000663.1_s304c1, BRDN0000402604
Derived from:
ccsbBroadEn_12453
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NECAB1 (64168)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12453
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64168 NECAB1 N-terminal EF-hand calcium ... NM_022351.5 28.3% 28.2% 1_753del;783A>G
2 human 64168 NECAB1 N-terminal EF-hand calcium ... XM_011517213.2 21.5% 18.5% (many diffs)
3 mouse 69352 Necab1 N-terminal EF-hand calcium ... NM_178617.4 25.6% 27.5% (many diffs)
4 mouse 69352 Necab1 N-terminal EF-hand calcium ... XM_006538234.1 24% 25.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
366
ORF length:
300
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct tgtgcagcgg cagatgtctg tgatggaaga ggacctggaa gaattccagc
121tcgctctgaa acactacgtg gagagtgctt cctcccaaag tggatgcttg cgtatttcta
181tacagaagct ttcaaatgaa tctcgctaca tgatctatga gttctgggag aatagtagtg
241tatggaatag ccaccttcag acaaattata gcaagacatt ccaaagaagt aatgtggatt
301tcttggaaac tccagaactc acatctacaa tgctagttcc tgcttcgtgg tggatcctga
361acaactaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
421gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
481acctctggat ta

Download FASTA (ORF) (Full)