Construct: ORF ccsbBroad304_12453
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000663.1_s304c1, BRDN0000402604
- Derived from:
- ccsbBroadEn_12453
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NECAB1 (64168)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12453
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64168 | NECAB1 | N-terminal EF-hand calcium ... | NM_022351.5 | 28.3% | 28.2% | 1_753del;783A>G |
| 2 | human | 64168 | NECAB1 | N-terminal EF-hand calcium ... | XM_011517213.2 | 21.5% | 18.5% | (many diffs) |
| 3 | mouse | 69352 | Necab1 | N-terminal EF-hand calcium ... | NM_178617.4 | 25.6% | 27.5% | (many diffs) |
| 4 | mouse | 69352 | Necab1 | N-terminal EF-hand calcium ... | XM_006538234.1 | 24% | 25.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 366
- ORF length:
- 300
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct tgtgcagcgg cagatgtctg tgatggaaga ggacctggaa gaattccagc 121 tcgctctgaa acactacgtg gagagtgctt cctcccaaag tggatgcttg cgtatttcta 181 tacagaagct ttcaaatgaa tctcgctaca tgatctatga gttctgggag aatagtagtg 241 tatggaatag ccaccttcag acaaattata gcaagacatt ccaaagaagt aatgtggatt 301 tcttggaaac tccagaactc acatctacaa tgctagttcc tgcttcgtgg tggatcctga 361 acaactaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 421 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 481 acctctggat ta