Construct: ORF ccsbBroad304_12523
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000816.1_s304c1, BRDN0000398679
- Derived from:
- ccsbBroadEn_12523
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OTUB2 (78990)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12523
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 78990 | OTUB2 | OTU deubiquitinase, ubiquit... | NM_023112.4 | 31.1% | 31.1% | 220_702del |
| 2 | mouse | 68149 | Otub2 | OTU domain, ubiquitin aldeh... | NM_026580.4 | 29% | 29.4% | (many diffs) |
| 3 | mouse | 68149 | Otub2 | OTU domain, ubiquitin aldeh... | XM_006516185.3 | 24.4% | 24.5% | (many diffs) |
| 4 | mouse | 68149 | Otub2 | OTU domain, ubiquitin aldeh... | NM_001177841.1 | 21% | 21% | (many diffs) |
| 5 | mouse | 68149 | Otub2 | OTU domain, ubiquitin aldeh... | NR_033587.1 | 6.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 285
- ORF length:
- 219
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag tgaaacatct ttcaacctaa tatcagaaaa atgtgacatt ctatccattc 121 ttcgggacca tcctgaaaac aggatttacc ggaggaaaat cgaggaactc agcaaaaggt 181 tcaccgccat ccgcaagacc aaaggggatg ggaactgctt ctacagggcc ttgggctatt 241 cctacctgga gtccctgctg gggaagagca gggagatctt caagtgccca actttcttgt 301 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 361 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a