Construct: ORF ccsbBroad304_12589

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014390.1_s304c1, BRDN0000409005
Derived from:
ccsbBroadEn_12589
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NLRX1 (79671)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12589
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79671 NLRX1 NLR family member X1 XM_005271670.1 84.5% 81.1% (many diffs)
2 human 79671 NLRX1 NLR family member X1 XM_005271671.1 84.5% 81.1% (many diffs)
3 human 79671 NLRX1 NLR family member X1 XM_005271672.1 84.5% 81.1% (many diffs)
4 human 79671 NLRX1 NLR family member X1 XM_006718904.1 75% 75% 1_687del;2378C>A
5 human 79671 NLRX1 NLR family member X1 NM_001282143.2 69.1% 66.3% (many diffs)
6 human 79671 NLRX1 NLR family member X1 NM_001282144.2 69.1% 66.3% (many diffs)
7 human 79671 NLRX1 NLR family member X1 NM_001282358.1 69.1% 66.3% (many diffs)
8 human 79671 NLRX1 NLR family member X1 NM_024618.4 69.1% 66.3% (many diffs)
9 human 79671 NLRX1 NLR family member X1 XM_005271669.1 69.1% 66.3% (many diffs)
10 human 79671 NLRX1 NLR family member X1 XM_011542980.1 69.1% 66.3% (many diffs)
11 human 79671 NLRX1 NLR family member X1 XM_017018294.2 35.9% 36.4% 1_687del;1670_1730del;1764_1765ins1060
12 mouse 270151 Nlrx1 NLR family member X1 NM_001163742.1 60.1% 61.5% (many diffs)
13 mouse 270151 Nlrx1 NLR family member X1 NM_001163743.1 60.1% 61.5% (many diffs)
14 mouse 270151 Nlrx1 NLR family member X1 NM_178420.3 60.1% 61.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2142
ORF length:
2076
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc tgctgctggg tcccacctcc tctttgtgct ccatggctta gagcatctca
121acctcgactt ccggctggca ggcacgggac tttgtagtga cccggaggaa ccgcaggaac
181cagctgctat catcgtcaac ctgctgcgca aatacatgct gcctcaggcc agcattctgg
241tgaccactcg gccctctgcc attggccgta tccccagcaa gtacgtgggc cgctatggtg
301agatctgcgg tttctctgat accaacctgc agaagctcta cttccagctc cgcctcaacc
361agccgtactg cgggtatgcc gttggcggtt caggtgtctc tgccacacca gctcagcgtg
421accacctggt gcagatgctc tcccggaacc tggaggggca ccaccagata gccgctgcct
481gcttcctgcc gtcctattgc tggctcgttt gtgccacctt gcacttcctg catgccccca
541cgcctgctgg gcagaccctt acaagcatct ataccagctt cctgcgcctc aacttcagcg
601gggaaaccct ggacagcact gacccctcca atttgtccct gatggcctat gcagcccgaa
661ccatgggcaa gttggcctat gagggggtgt cctcccgcaa gacctacttc tctgaagagg
721atgtctgtgg ctgcctggag gctggcatca ggacggagga ggagtttcag ctgctgcaca
781tcttccgtcg ggatgccctg aggtttttcc tggccccatg tgtggagcca gggcgtgcag
841gcaccttcgt gttcaccgtg cccgccatgc aggaatacct ggctgccctc tacattgtgc
901tgggtttgcg caagacgacc ctgcaaaagg tgggcaagga agtggctgag ctcgtgggcc
961gtgttgggga ggacgtcagc ctggtactgg gcatcatggc caagctgctg cctctgcggg
1021ctctgcctct gctcttcaac ctgatcaagg tggttccacg agtgtttggg cgcatggtgg
1081gtaaaagccg ggaggcggtg gctcaggcca tggtgctgga gatgtttcga gaggaggact
1141actacaacga tgatgttctg gaccagatgg gcgccagtat cctgggcgtg gagggccccc
1201ggcgccaccc agatgagccc cctgaggatg aagtcttcga gctcttcccc atgttcatgg
1261gggggcttct ctctgcccac aaccgagctg tgctagctca gcttggctgc cccatcaaga
1321acctggatgc cctggagaat gcccaggcca tcaagaagaa gctgggcaag ctgggccggc
1381aggtgctgcc cccatcagag ctccttgacc acctcttctt ccactatgag ttccagaacc
1441agcgcttctc cgctgaggtg ctcagctccc tgcgtcagct caacctggca ggtgtgcgca
1501tgacaccagt caagtgcaca gtggtggcag ctgtgctggg cagcggaagg catgccctgg
1561atgaggtgaa cttggcctcc tgccagctag atcctgctgg gctgcgcaca ctcctgcctg
1621tcttcctgcg tgcccggaag ctgggcttgc aactcaacag cctgggccct gaggcctgca
1681aggacctccg agacctgttg ctgcatgacc agtgccaaat taccacactg cggctgtcca
1741acaacccgct gacggaggca ggtgttgccg tgctaatgga ggggctggca ggaaacacct
1801cagtgacgca cctgtccctg ctgcacacgg gccttgggga cgaaggcctg gagctgctgg
1861ctgcccagct ggaccgcaac cggcagctgc aggagctgaa cgtggcgtac aacggtgctg
1921gtgacacagc ggccctggcc ctggccagag ctgcccggga gcacccttcc ctggaactgc
1981tacagggtgt cgccatccag atgtgttgga agcttcccct cctgccttat gctcacctgt
2041ggacaccgag gatgccctca cattggtgct ttctcctcat cctcatgccc cctttgccac
2101aatggtatga tggcttggta gcccctcgag gcagatgcac ctgcccaact ttcttgtaca
2161aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
2221ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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